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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleMolecular characterization of a new arcelin-5 genept_BR
dc.contributor.authorGerhardt, IRpt_BR
dc.contributor.authorPaes, NSpt_BR
dc.contributor.authorBloch, Cpt_BR
dc.contributor.authorMendes, PAMpt_BR
dc.contributor.authorLeite, Apt_BR
dc.contributor.authorChrispeels, MJpt_BR
dc.contributor.authorde Sa, MFGpt_BR
unicamp.authorEMBRAPA, Cenargen, Brasilia, DF, Brazil UnB, Dept Biol Celular, Brasilia, DF, Brazil UNICAMP, CBMEG, Campinas, SP, Brazil UCSD, Dept Biol, La Jolla, CA USApt_BR
dc.subjectarcelin-5pt_BR
dc.subjectinsect inhibitory activitypt_BR
dc.subjectseed storage proteinpt_BR
dc.subjectmass spectrometrypt_BR
dc.subject.wosSeed Proteinpt_BR
dc.subject.wosPhaseolus-vulgarispt_BR
dc.subject.wosPhytohemagglutininpt_BR
dc.subject.wosInheritancept_BR
dc.subject.wosResistancept_BR
dc.subject.wosVariantspt_BR
dc.subject.wosCleavagept_BR
dc.subject.wosBindingpt_BR
dc.subject.wosWeevilpt_BR
dc.description.abstractArcelins are insecticidal proteins found in some wild accessions of the common bean, Phaseolus vulgaris. They are grouped in six allelic variants and arcelin-5 is the variant with the highest inhibitory effect on the development of Zabrotes subfasciatus larvae. Characterization of the protein and its genes resulted in the identification of three polypeptides and the isolation of two genes that encode the Arc5a and Arc5b polypeptides. Here we describe a new gene, Arc5-III. The protein it encodes has 81% amino acid identity with the derived amino acid sequences of Arc5-I and Arc5-II. The Arc5-III gene is highly expressed in developing seeds and at a much lower level in roots. Data obtained by a combination of two-dimensional gel electrophoresis, protein sequencing and MALDI-TOF mass spectrometry analysis support the conclusion that Arc5-III encodes a polypeptide present in Arc5c band, Using ion-exchange chromatography, three fractions containing arcelin-5 polypeptides were eluted by increasing the salt concentration. The three fractions contain various amounts of the three arc-5 polypeptides and inhibit the growth of Zabrotes subfasciatus larvae differentially, suggesting differences in insecticidal activity among the arcelin-5 isoforms. (C) 2000 Elsevier Science B.V. All rights reserved.pt
dc.relation.ispartofBiochimica Et Biophysica Acta-gene Structure And Expressionpt_BR
dc.relation.ispartofabbreviationBiochim. Biophys. Acta-Gene Struct. Expressionpt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryHolandapt_BR
dc.publisherElsevier Science Bvpt_BR
dc.date.issued2000pt_BR
dc.date.monthofcirculation11324pt_BR
dc.identifier.citationBiochimica Et Biophysica Acta-gene Structure And Expression. Elsevier Science Bv, v. 1490, n. 41671, n. 87, n. 98, 2000.pt_BR
dc.language.isoenpt_BR
dc.description.volume1490pt_BR
dc.description.issuenumber41671pt_BR
dc.description.firstpage87pt_BR
dc.description.lastpage98pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0167-4781pt_BR
dc.identifier.wosidWOS:000085218300009pt_BR
dc.identifier.doi10.1016/S0167-4781(99)00219-5pt_BR
dc.date.available2014-12-02T16:24:42Z
dc.date.available2015-11-26T17:18:39Z-
dc.date.accessioned2014-12-02T16:24:42Z
dc.date.accessioned2015-11-26T17:18:39Z-
dc.description.provenanceMade available in DSpace on 2014-12-02T16:24:42Z (GMT). No. of bitstreams: 1 WOS000085218300009.pdf: 1496218 bytes, checksum: 6516cddcb5af5fd6ab4f08c77df29c0f (MD5) Previous issue date: 2000en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:18:39Z (GMT). No. of bitstreams: 2 WOS000085218300009.pdf: 1496218 bytes, checksum: 6516cddcb5af5fd6ab4f08c77df29c0f (MD5) WOS000085218300009.pdf.txt: 35882 bytes, checksum: f080caaee0884d4cd0e6e8f76dc1dd39 (MD5) Previous issue date: 2000en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57733pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/57733
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57733-
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