Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/576
Type: Artigo de periódico
Title: Monoamine oxidase inhibitory activities of indolylalkaloid toxins from the venom of the colonial spider Parawixia bistriata: Functional characterization of PwTX-I
Author: SAIDEMBERG, Daniel M.
FERREIRA, Marco A. B.
TAKAHASHI, Tatiane N.
GOMES, Paulo C.
CESAR-TOGNOLI, Lilian M. M.
SILVA-FILHO, Luiz C. da
TORMENA, Claudio F.
SILVA, Gil V. J. da
PALMA, Mario S.
Abstract: Colonial spiders evolved a differential prey-capture behaviour in concert with their venom chemistry, which may be a source of novel drugs. Some highly active tetrahydro-beta-carboline (TH beta C) toxins were recently isolated from the venom of the colonial spider Parawixia bistriata; the spiders use these toxins as part of their chemical arsenal to kill and/or paralyze preys. The major TH beta C compound isolated from this venom was identified as 6-hydroxytrypargine, also known as PwTX-I. Most natural compounds of animal origin occur in low abundance, and the natural abundance of PwTX-I is insufficient for complete functional characterization. Thus, PwTx-I was synthesized using a Pictet-Spengler condensation strategy, and the stereoisomers of the synthetic toxin were separated by chiral chromatography. The fraction of venom containing a mixture of three natural TH beta C toxins and enantiomers of PwTx-I were analyzed for inhibition of monoamine oxidase (MAO)-A and -B and for toxicity to insects. We reveal that the mixture of the natural TH beta C toxins, as well as the enantiomers of PwTx-I, were non-competitive inhibitors of MAO-A and MAO-B and caused potent paralysis of honeybees. The (-)-PwTX-I enantiomer is 2-fold more potent than the (+)-PwTX-I enantiomer in the assays performed. (C) 2009 Elsevier Ltd. All rights reserved.
Subject: Spider venom
Parawixiatoxin
Indoylalkaloid
Chiral chromatography
Monoamine oxidase
Country: Inglaterra
Editor: PERGAMON-ELSEVIER SCIENCE LTD
Citation: TOXICON, v.54, n.6, p.717-724, 2009
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2009.05.027
Address: http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000270626900002&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
http://dx.doi.org/10.1016/j.toxicon.2009.05.027
Date Issue: 2009
Appears in Collections:IQ - Artigos e Outros Documentos

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