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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleChromatin supraorganization and extensibility in mouse hepatocytes following starvation and refeedingpt_BR
dc.contributor.authorMoraes, ASpt_BR
dc.contributor.authorVidal, BDpt_BR
dc.contributor.authorGuaraldo, AMApt_BR
dc.contributor.authorMello, MLSpt_BR
unicamp.author.emailmlsmello@unicamp.brpt_BR
unicamp.authorUniv Estadual Campinas, UNICAMP, Inst Biol, Dept Cell Biol, BR-13083863 Campinas, SP, Brazil Univ Estadual Campinas, UNICAMP, Multidisciplinary Ctr Biol Invest, BR-13083863 Campinas, SP, Brazilpt_BR
dc.subjectchromatin supraorganizationpt_BR
dc.subjectextended chromatin fiberspt_BR
dc.subjectstarvationpt_BR
dc.subjectrefeedingpt_BR
dc.subjectnuclear matrixpt_BR
dc.subject.wosIn-situ Hybridizationpt_BR
dc.subject.wosNuclear-matrixpt_BR
dc.subject.wosExtended Chromatinpt_BR
dc.subject.wosConcanavalin-apt_BR
dc.subject.wosGene-expressionpt_BR
dc.subject.wosHistone Contentpt_BR
dc.subject.wosRat-liverpt_BR
dc.subject.wosProteinspt_BR
dc.subject.wosCellspt_BR
dc.subject.wosDnapt_BR
dc.description.abstractBackground: The effect of 48 h of starvation and of 48 h of refeeding subsequent to starvation on chromatin supraorganization and extensibility was studied in hepatocytes from adult mice. Methods: Methods used involved topochemical assays, image analysis, gravity action, and polarization microscopy. Results: Starvation increased the chromatin packing states, especially in areas of noncondensed chromatin, and induced drastic decreases in concanavalin A reactivity due to nuclear matrix glycoproteins and the frequency of nuclei with chromatin extensibility under gravity. Changes in chromatin packing state were accompanied by shifts of nuclear areas of part of the nuclear population to smaller values but did not affect the respective Feulgen-DNA amounts except for a few nuclei. The extent of chromatin unpackaging, but not of frequency of nuclei with formation of extended chromatin fibers, in starved mice that were refed was greater than in well-fed controls. Refeeding induced increase in Feulgen-DNA amounts and regain and redistribution of concanavalin A-reactive nuclear glycoproteins. However, the duration of refeeding used was probably insufficient to reestablish the stereo arrangement of the chromatin-nuclear matrix and to restore chromatin fluidity to the level seen in well-fed mice. Conclusions: The changes in the liver cell nuclei associated with starvation and refeeding of adult mice involved chromatin supraorganization, hepatocyte proliferation (refeeding), and the loss, regain, and redistribution of nuclear proteins, especially nuclear matrix components, related to chromatin organization and extensibility. These changes are suggested as favoring the silencing and reactivation of transcriptional activities, depending on the organism's nutritional state. (C) 2005 Wiley-Liss, Inc.pt
dc.relation.ispartofCytometry Part Apt_BR
dc.relation.ispartofabbreviationCytom. Part Apt_BR
dc.publisher.cityHobokenpt_BR
dc.publisher.countryEUApt_BR
dc.publisherWiley-lisspt_BR
dc.date.issued2005pt_BR
dc.date.monthofcirculationFEBpt_BR
dc.identifier.citationCytometry Part A. Wiley-liss, v. 63A, n. 2, n. 94, n. 107, 2005.pt_BR
dc.language.isoenpt_BR
dc.description.volume63Apt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage94pt_BR
dc.description.lastpage107pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlpt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1552-4922pt_BR
dc.identifier.wosidWOS:000226858100005pt_BR
dc.identifier.doi10.1002/cyto.a.20113pt_BR
dc.date.available2014-11-15T18:56:45Z
dc.date.available2015-11-26T16:13:11Z-
dc.date.accessioned2014-11-15T18:56:45Z
dc.date.accessioned2015-11-26T16:13:11Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:13:11Z (GMT). No. of bitstreams: 2 WOS000226858100005.pdf: 644172 bytes, checksum: 85c2492824a89e88534a696f37c63949 (MD5) WOS000226858100005.pdf.txt: 44701 bytes, checksum: 20a1ba0dace3e09f55658e0508668272 (MD5) Previous issue date: 2005en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55842pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/55842
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55842-
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