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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleBinding motifs of CBP2 a potential cell surface target for carcinoma cellspt_BR
dc.contributor.authorSauk, JJpt_BR
dc.contributor.authorColetta, RDpt_BR
dc.contributor.authorNorris, Kpt_BR
dc.contributor.authorHebert, Cpt_BR
unicamp.authorUniv Maryland, Sch Dent, Baltimore, MD 21201 USA Univ Maryland, UMB Greenebaum Canc Ctr, Baltimore, MD 21201 USA Univ Maryland, Dept Pathol, Baltimore, MD 21201 USA Univ Campinas, Dept Oral Pathol, Sao Paulo, Brazilpt_BR
dc.subjectCBP2pt_BR
dc.subjectHsp47pt_BR
dc.subjectcarcinomapt_BR
dc.subjectbacteriophagept_BR
dc.subjecttargetingpt_BR
dc.subject.wosCarboxyl-terminal Propeptidept_BR
dc.subject.wosMolecular Chaperone Hsp47pt_BR
dc.subject.wosSarcoma-180 In-vivopt_BR
dc.subject.wosShock Protein Hsp47pt_BR
dc.subject.wosOsteogenesis Imperfectapt_BR
dc.subject.wosEndoplasmic-reticulumpt_BR
dc.subject.wosPeptide Librariespt_BR
dc.subject.wosKdel Receptorpt_BR
dc.subject.wosDecreased Expressionpt_BR
dc.subject.wosProcollagenpt_BR
dc.description.abstractPreviously we have shown (Hebert et al. [1999] J. Cell Biochem. 73:248-258) that among many cell lines the CBP2 gene product, Hsp47, eludes its retention receptor, erd2P, resulting in the appearance of Hsp47 on the cell surface associated with the tetraspanin protein CD9. Since Hsp47 possesses a highly restricted binding cleft, random peptide display libraries were used to characterize peptides binding to Hsp47 and then to target this protein on carcinoma cell lines in vitro. Comparison of the clones obtained from panning revealed little specific homology based on sequence alone. To determine whether carcinoma cells expressing Hsp47 could selectively take up the selected bacteriophages, traditional immunofluorescence and confocal microscopy were employed. These studies revealed that phage-displaying Hsp47 binding peptides bound to cell lines expressing Hsp47 and that the peptides were rapidly taken up to a location coincident with Hsp47 staining. These observations were confirmed by cytometric analyses. These data indicate that CBP2 product may provide a molecular target for chemotherapy and/or imaging of malignancies. (C) 2000 Wiley-Liss, Inc.pt
dc.relation.ispartofJournal Of Cellular Biochemistrypt_BR
dc.relation.ispartofabbreviationJ. Cell. Biochem.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisher.countryEUApt_BR
dc.publisherWiley-lisspt_BR
dc.date.issued2000pt_BR
dc.date.monthofcirculationMAYpt_BR
dc.identifier.citationJournal Of Cellular Biochemistry. Wiley-liss, v. 78, n. 2, n. 251, n. 263, 2000.pt_BR
dc.language.isoenpt_BR
dc.description.volume78pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage251pt_BR
dc.description.lastpage263pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlpt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0730-2312pt_BR
dc.identifier.wosidWOS:000087656900008pt_BR
dc.identifier.doi10.1002/(SICI)1097-4644(20000801)78:2<251pt_BR
dc.date.available2014-07-30T13:51:56Z
dc.date.available2015-11-26T17:10:26Z-
dc.date.accessioned2014-07-30T13:51:56Z
dc.date.accessioned2015-11-26T17:10:26Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T13:51:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2000en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:10:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2000en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55477
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55477-
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