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Type: Artigo de periódico
Title: ARHGAP21 Protein, a New Partner of alpha-Tubulin Involved in Cell-Cell Adhesion Formation and Essential for Epithelial-Mesenchymal Transition
Author: Barcellos, KSA
Bigarella, CL
Wagner, MV
Vieira, KP
Lazarini, M
Langford, PR
Machado-Neto, JA
Call, SG
Staley, DM
Chung, JY
Hansen, MD
Saad, STO
Abstract: Cell-cell adhesions and the cytoskeletons play important and coordinated roles in cell biology, including cell differentiation, development, and migration. Adhesion and cytoskeletal dynamics are regulated by Rho-GTPases. ARHGAP21 is a negative regulator of Rho-GTPases, particularly Cdc42. Here we assess the function ofARHGAP21in cell-cell adhesion, cell migration, and scattering. We find that ARHGAP21 is localized in the nucleus, cytoplasm, or perinuclear region but is transiently redistributed to cell-cell junctions 4 h after initiation of cell-cell adhesion. ARHGAP21 interacts with Cdc42, and decreased Cdc42 activity coincides with the appearance of ARHGAP21 at the cell-cell junctions. Cells lacking ARHGAP21 expression show weaker cell-cell adhesions, increased cell migration, and a diminished ability to undergo hepatocyte growth factor-induced epithelial-mesenchymal transition (EMT). In addition, ARHGAP21 interacts with alpha-tubulin, and it is essential for alpha-tubulin acetylation in EMT. Our findings indicate that ARHGAP21 is a Rho-GAP involved in cell-cell junction remodeling and that ARHGAP21 affects migration and EMT through alpha-tubulin interaction and acetylation.
Country: EUA
Editor: Amer Soc Biochemistry Molecular Biology Inc
Citation: Journal Of Biological Chemistry. Amer Soc Biochemistry Molecular Biology Inc, v. 288, n. 4, n. 2179, n. 2189, 2013.
Rights: fechado
Identifier DOI: 10.1074/jbc.M112.432716
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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