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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleA comparison between IgG antibodies against Eimeria acervulina, E-maxima, and E-tenella and oocyst shedding in broiler-breeders vaccinated with live anticoccidial vaccinespt_BR
dc.contributor.authorGuzman, VBpt_BR
dc.contributor.authorSilva, DAOpt_BR
dc.contributor.authorKawazoe, Upt_BR
dc.contributor.authorMineo, JRpt_BR
unicamp.authorUniv Fed Uberlandia, Dept Immunol Microbiol & Parasitol, Immunol Lab, BR-38400902 Uberlandia, MG, Brazil Univ Campinas, Dept Parasitol, Lab Avian Coccidiosis, Campinas, SP, Brazilpt_BR
dc.subjectE. acervulinapt_BR
dc.subjectE. maximapt_BR
dc.subjectE. tenellapt_BR
dc.subjectlive anticoccidial vaccinespt_BR
dc.subjectIgG antibodiespt_BR
dc.subjectoocyst sheddingpt_BR
dc.subject.wosAvian Coccidiosispt_BR
dc.subject.wosProtective Immunitypt_BR
dc.subject.wosChickenspt_BR
dc.subject.wosParasitespt_BR
dc.subject.wosTransmissionpt_BR
dc.subject.wosResponsespt_BR
dc.subject.wosInfectionpt_BR
dc.subject.wosAntigenspt_BR
dc.subject.wosExposurept_BR
dc.description.abstractThe aim of this study was to compare the IgG response against Eimeria acervulina, E. maxima, and E. tenella and oocyst shedding in broiler-breeders vaccinated with live (virulent or attenuated) anticoccidial vaccines. Serum and faeces samples were collected from chickens before (4 days of age) and after (25 days of age) vaccination with virulent (group A) or attenuated (group B) vaccines and unvaccinated control birds (group Q. Criteria for evaluation included the number of oocysts shed on the litter (McMaster technique), the levels of specific serum IgG (ELISA) and the frequencies of antigenic fractions recognised by IgG antibodies (immunoblotting). The results demonstrated that the virulent vaccine (A) presented an ideal association (low oocyst number and high IgG positive rate) for E. acervulina and E. tenella while the immunised flock with the attenuated vaccine (B) demonstrated this ideal association only for E. maxima. Both vaccinated flocks demonstrated a better association for three Eimeria spp. studied when compared to the control group, which presented a divergent association (high oocyst number and low IgG positive rate), particularly for E. acervulina. Immumoblotting assays revealed different antigenic fractions that may be considered as inummodominant antigens. The present study suggests that E. acervulina is critically present in the region studied and the use of live anticoccidial vaccines (virulent or attenuated) showed to be effective to control the clinical coccidiosis in vaccinated flocks. (C) 2003 Elsevier Ltd. All rights reserved.pt
dc.relation.ispartofVaccinept_BR
dc.relation.ispartofabbreviationVaccinept_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherElsevier Sci Ltdpt_BR
dc.date.issued2003pt_BR
dc.date.monthofcirculationOCT 1pt_BR
dc.identifier.citationVaccine. Elsevier Sci Ltd, v. 21, n. 27-30, n. 4225, n. 4233, 2003.pt_BR
dc.language.isoenpt_BR
dc.description.volume21pt_BR
dc.description.issuenumber27-30pt_BR
dc.description.firstpage4225pt_BR
dc.description.lastpage4233pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0264-410Xpt_BR
dc.identifier.wosidWOS:000186830800018pt_BR
dc.identifier.doi10.1016/S0264-410X(03)00462-6pt_BR
dc.date.available2014-11-19T03:47:09Z
dc.date.available2015-11-26T17:56:58Z-
dc.date.accessioned2014-11-19T03:47:09Z
dc.date.accessioned2015-11-26T17:56:58Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T17:56:58Z (GMT). No. of bitstreams: 2 WOS000186830800018.pdf: 187040 bytes, checksum: 1eafbf8c388842300fbff6362c72017e (MD5) WOS000186830800018.pdf.txt: 37382 bytes, checksum: 9fa979ec314f0250f74c1c8a7932fd3d (MD5) Previous issue date: 2003en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/52985pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/52985
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/52985-
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