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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleLow-protein diets reduce PKA alpha expression in islets from pregnant ratspt_BR
dc.contributor.authorMilanski, Mpt_BR
dc.contributor.authorArantes, VCpt_BR
dc.contributor.authorFerreira, Fpt_BR
dc.contributor.authorReis, MADpt_BR
dc.contributor.authorCarneiro, EMpt_BR
dc.contributor.authorBoschero, ACpt_BR
dc.contributor.authorCollares-Buzato, CBpt_BR
dc.contributor.authorLatorraca, MQpt_BR
unicamp.authorUniv Fed Mato Grosso, Fac Nutr, Dept Alimentos & Nutr, Cuiaba, MT, Brazil Secretaria Estado Saude Sao Paulo, Mato Grosso, MT, Brazil Univ Fed Pernambuco, Dept Fisiol & Farmacol, Recife, PE, Brazil Univ Estadual Campinas, Dept Fisiol & Biofis, Inst Biol, Campinas, SP, Brazil Univ Estadual Campinas, Dept Histol & Embryol, Inst Biol, Campinas, SP, Brazilpt_BR
dc.subjectlow-protein dietpt_BR
dc.subjectinsulin secretionpt_BR
dc.subjectPKA alphapt_BR
dc.subjectPKC alphapt_BR
dc.subject.wosInduced Insulin-releasept_BR
dc.subject.wosPancreatic Beta-cellspt_BR
dc.subject.wosLactogenic Hormonespt_BR
dc.description.abstractWe investigated the effect of protein restriction on insulin secretion and the expression of protein kinase (PK)A alpha and PKC alpha in islets from control and pregnant rats. Adult control nonpregnant (CN) and control pregnant (CP) rats were fed a normal-protein diet (17%), whereas low-protein nonpregnant (LPN) and low-protein pregnant (LPP) rats were fed a low-protein diet (6%) for 15 d. In the presence of 2.8 and 8.3 mmol glucose/L, insulin secretion by islets of CP rats was higher than that by islets of CN rats. Compared with the CN groups, insulin secretion by islets of LPN rats was lower with 8.3 but not with 2.8 mmol glucose/L. The insulin secretion by islets of LPP rats was higher than by LPN rats at both glucose concentrations. IBMX (1 mmol/L), a phosphodiesterase inhibitor, increased insulin secretion by islets from pregnant rats, and this effect was greater in islets of CP rats than in LPP rats. Forskolin (0.01 -100 mu mol/L), a stimulator of adenylyl cyclase, increased insulin sacretion only in islets of CN and CP rats, with a higher 50% effective concentration in islets of CP rats compared with CN rats. The insulin secretion induced by phorbol 12-myristate 13-acetate (a stimulator of PKC) was higher in islets of LPN and LPP rats than in the respective controls, especially, at 8.3 mmol glucose/L. PKA alpha, but not PKC alpha, expression was lower in islets of rats fed low protein than in the controls, regardless of the physiological status of the rats. All endocrine cells of the islets, including beta-cells, expressed the PKA alpha isoform. The cytoplasmic distribution of this enzyme in beta-cells was not modified by pregnancy and/or protein restriction. In conclusion, our results indicate that the response of islets from rats fed low protein during pregnancy is similar to that of control rats, at least for physiologic glucose concentration. However, the decreased response to IBMX and forskolin indicates decreased production and/or sensitivity to cAMP; this was associated with a decrease in PKA expression, which may result in lower PKA
dc.relation.ispartofJournal Of Nutritionpt_BR
dc.relation.ispartofabbreviationJ. Nutr.pt_BR
dc.publisherAmer Society Nutritional Sciencept_BR
dc.identifier.citationJournal Of Nutrition. Amer Society Nutritional Science, v. 135, n. 8, n. 1873, n. 1878, 2005.pt_BR
dc.sourceWeb of Sciencept_BR
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