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Type: Artigo de periódico
Title: Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation
Author: Leite, Nadjma Souza
Lima, Aloísio André Bomfim de
Santana, José Carlos Curvelo
Lopes, Francisco Luiz Gumes
Lédo, Ana da Silva
Tambourgi, Elias Basile
Souza, Roberto Rodrigues de
Abstract: This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL). Imperial cultivar showed higher bromelain activity than the Pérola's.
Subject: Enzymatic activity
Pérola pineapple
Imperial pineapple
production in vitro
Editor: Instituto de Tecnologia do Paraná - Tecpar
Citation: Brazilian Archives of Biology and Technology. Instituto de Tecnologia do Paraná - Tecpar, v. 55, n. 5, p. 647-652, 2012.
Rights: aberto
Identifier DOI: 10.1590/S1516-89132012000500002
Date Issue: 1-Oct-2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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