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Type: Artigo de periódico
Title: The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS
Author: Ferreira, Juliana Ferrari
Santana, José Carlos Curvelo
Tambourgi, Elias Basile
Abstract: In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain was purified. Optimum conditions of bromelain use were found at pH 7 and between 30-40ºC.
Subject: bromelain
aqueous two-phase systems
Editor: Instituto de Tecnologia do Paraná - Tecpar
Citation: Brazilian Archives of Biology and Technology. Instituto de Tecnologia do Paraná - Tecpar, v. 54, n. 1, p. 125-132, 2011.
Rights: aberto
Identifier DOI: 10.1590/S1516-89132011000100017
Date Issue: 1-Feb-2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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