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Type: Pré-print
Title: Compartmentalization of therapeutic proteins into semi-crystalline PEG-PCL polymersomes
Author: Pachioni-Vasconcelos, Juliana de Almeida
Apolinário, Alexsandra Conceição
Lopes, André Moreni
Pessoa, Adalberto
Barbosa, Leandro Ramos Souza
Rangel-Yagui, Carlota de Oliveira
Abstract: Polymersomes are self-assembled nanostructures with high loading capacity, possibility to deliver hydrophilic as well as hydrophobic drugs and stealth characteristic resulting in low immunogenicity. These vesicles can be used to deliver enzyme drugs such as L-asparaginase (ASNase), a first line drug for acute lymphoblastic leukemia treatment. Here, polymersomes based on three semi-crystalline copolymers of poly(ethylene glycol)-b-poly(ε-caprolactone), namely PEG45PCL44, PEG114PCL98, and PEG114PCL114, were investigated for the encapsulation of ASNase, as well as of bovine serum albumin (BSA) as a model protein. Critical aggregation concentration (CAC) of the copolymers was determined by fluorescence spectroscopy and the values varied from 0.6 to 1.26 mg/L. Using film hydration, polymersomes of 200–400 nm and narrow size distribution (polydispersity index values of 0.2–0.3) were obtained when centrifugation was used as a post-film technique. The encapsulation efficiency (EE %) was determined either after centrifugation of the suspension, followed by the proteins measurement in the supernatant or after Size Exclusion Chromatography (SEC) purification, in which the quantification was performed in the eluted fractions corresponding to the free protein. Higher encapsulation efficiency values were obtained after centrifugation (EE% ≈ 20%) in comparison to the measurements after SEC (EE% ≈ 1–5%), indicating that the protein could be partially entrapped in the polymeric network when centrifugation is used as separation method. Nonetheless, this system could provide an initial effect of the free ASNase followed by a long-term effect based on the encapsulated enzyme, leading to decreased dose administration
Subject: Sistemas de distribuição de medicamentos
Country: Estados Unidos
Editor: Taylor & Francis
Rights: Fechado
Identifier DOI: 10.1080/1539445X.2020.1812643
Date Issue: 2020
Appears in Collections:FCF - Artigos e Outros Documentos

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