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Type: Artigo
Title: Effect of tyrosol on adhesion of Candida albicans and Candida glabrata to acrylic surfaces
Author: Monteiro, Douglas Roberto
Feresin, Leonardo Perina
Arias, Laís Salomão
Barão, Valentim Adelino Ricardo
Barbosa, Debora Barros
Delbem, Alberto Carlos Botazzo
Abstract: The prevention of adhesion of Candida cells to acrylic surfaces can be regarded as an alternative to prevent denture stomatitis. The use of quorum sensing molecules, such as tyrosol, could potentially interfere with the adhesion process. Therefore, the aim of this study was to assess the effect of tyrosol on adhesion of single and mixed cultures of Candida albicans and Candida glabrata to acrylic resin surfaces. Tyrosol was diluted in each yeast inoculum (107 cells/ml in artificial saliva) at 25, 50, 100, and 200 mM. Then, each dilution was added to wells of 24-well plates containing the acrylic specimens, and the plates were incubated at 37°C for 2 h. After, the effect of tyrosol was determined by total biomass quantification, metabolic activity of the cells and colony-forming unit counting. Chlorhexidine gluconate (CHG) was used as a positive control. Data were analyzed using analysis of variance (ANOVA) and the Holm–Sidak post hoc test (α = 0.05). The results of total biomass quantification and metabolic activity revealed that the tyrosol promoted significant reductions (ranging from 22.32 to 86.16%) on single C. albicans and mixed cultures. Moreover, tyrosol at 200 mM and CHG significantly reduced (p < 0.05) the number of adhered cells to the acrylic surface for single and mixed cultures of both species, with reductions ranging from 1.74 to 3.64-log10. In conclusion, tyrosol has an inhibitory effect on Candida adhesion to acrylic resin, and further investigations are warranted to clarify its potential against Candida infections
Subject: Candida albicans
Country: Reino Unido
Editor: Oxford University Press
Rights: Fechado
Identifier DOI: 10.1093/mmy/myv052
Date Issue: 2015
Appears in Collections:FOP - Artigos e Outros Documentos

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