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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampVicentini, Renato-
dc.typeArtigopt_BR
dc.titleDifferential sRNA regulation in leaves and roots of sugarcane under water depletionpt_BR
dc.contributor.authorVieira, T.-
dc.contributor.authorCarnavale-Bottino, M.-
dc.contributor.authorTanurdzic, M.-
dc.contributor.authorGrativol, C.-
dc.contributor.authorThiebaut, F.-
dc.contributor.authorMotta, M. R.-
dc.contributor.authorHemerly, A. S.-
dc.contributor.authorVincentini, R.-
dc.contributor.authorRojas, C.-
dc.contributor.authorChabregas, S. M.-
dc.contributor.authorFerreira, P. C. G.-
dc.contributor.authorMartienssen, R. A.-
dc.subjectMetilação de DNApt_BR
dc.subjectArabidopsispt_BR
dc.subject.otherlanguageDNA Methylationpt_BR
dc.subject.otherlanguageArabidopsispt_BR
dc.description.abstractPlants have developed multiple regulatory mechanisms to respond and adapt to stress. Drought stress is one of the major constraints to agricultural productivity worldwide and recent reports have highlighted the importance of plant sRNA in the response and adaptation to water availability. In order to increase our understanding of the roles of sRNA in response to water depletion, cultivars of sugarcane were submitted to treatment of ceasing drip irrigation for 24 hours. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. The pool of sRNA selected allowed the analysis of different sRNA classes ( miRNA and siRNA). Twenty-eight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profiles of eight miRNA were verified in leaf samples from three biological replicates by stem-loop qRT-PCR assay using the cultivars: SP90-1638-sensitive cultivar- and; SP83-2847 and SP83-5073 tolerant cultivars. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. Two leaf libraries from individual sugarcane cultivars with contrasting drought-tolerance properties were also analyzed. An enrichment of 22-nt sRNA species was observed in leaf libraries. 22-nt miRNA triggered siRNA production by cleavage of their targets in response to water depletion. A number of genes of the sRNA biogenesis pathway were down-regulated in tolerant genotypes and up-regulated in sensitive in response to water depletion treatment. Our analysis contributes to increase the knowledge on the roles of sRNA in sugarcane submitted to water depletionpt_BR
dc.relation.ispartofPLoS onept_BR
dc.publisher.citySan Francisco, CApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherPublic Library of Sciencept_BR
dc.date.issued2014-
dc.date.monthofcirculationApr.pt_BR
dc.language.isoengpt_BR
dc.description.volume9pt_BR
dc.description.issuenumber4pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1932-6203pt_BR
dc.identifier.doi10.1371/journal.pone.0093822pt_BR
dc.identifier.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0093822pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO CARLOS CHAGAS FILHO DE AMPARO À PESQUISA DO ESTADO DO RIO DE JANEIRO - FAPERJpt_BR
dc.date.available2020-11-05T15:46:04Z-
dc.date.accessioned2020-11-05T15:46:04Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2020-11-05T15:46:04Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-16T16:52:51Z : No. of bitstreams: 1 000334103000126.pdf: 2578300 bytes, checksum: 7df110e7f6d49e8d59212fa67bffeeaa (MD5)en
dc.description.provenanceMade available in DSpace on 2020-11-05T15:46:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/351930-
dc.contributor.unidadeCentro de Biologia Molecular e Engenharia Genéticapt_BR
dc.identifier.source000334103000126pt_BR
dc.creator.orcid0000-0002-0527-1083pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.identifier.articleide93822pt_BR
dc.description.sponsorNoteInstituto Nacional de Ciencia e Tecnologia em Fixacao Biologica de Nitrogenio (INCT)pt_BR
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