Use este identificador para citar ou linkar para este item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/350151
Tipo: Artigo
Título: Increased glutathione levels contribute to the beneficial effects of hydrogen sulfide and inducible nitric oxide inhibition in allergic lung inflammation
Autor(es): Campos, Daiana
Ravagnani, Felipe G.
Gurgueira, Sonia A.
Vercesi, Anibal E.
Teixeira, Simone A.
Costa, Soraia K.P.
Muscará, Marcelo N.
Ferreira, Heloisa H.A.
Resumo: The interaction between nitric oxide (NO) and hydrogen sulfide (H2S) in the airways could have significant implications for the pathogenesis and therapeutic effects of both on lung diseases. In this study we investigated whether the beneficial effects of H2S on asthma could be comparable to that inhibition of inducible NO synthase (iNOS). Female BALB/C mice sensitized with ovalbumin (OVA) received either the H2S donor sodium hydrosulfide (NaHS, 14 μmol/kg) or the iNOS inhibitor 1400 W (1 mg/kg), 30 min before each OVA challenge during six days. On the first, second and sixth days, the leucocyte infiltration in lung parenchyma and bronchoalveolar lavage was evaluated. The aconitase activity (a sensor of O2radical dotsingle bond formation) and lipid peroxidation, as well as levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) were determined in the lung tissues. OVA-challenge caused a significant and time-dependent increase in the eosinophil number in the airways, which was accompanied by a significant decrease of aconitase activity and GSH/GSSG ratio along with enhanced lipid peroxidation in the lungs. Treatment with NaHS or 1400 W significantly attenuated the airways eosinophilia that was paralleled by an increase in aconitase activity and decrease of lipid peroxidation. NaHS or 1400 W treatments also reversed the decreased GSH/GSSG ratio seen after OVA-challenge. The present study shows for the first time that the increased GSH/GSSG ratio caused by either H2S supplementation or iNOS-inhibition is a potential mechanism protecting airways against oxidative stress and inflammatory lung diseases
Palavras-chave: Razão glutationa reduzida/oxidada
País: Reino Unido
Editor: Elsevier
Tipo de Acesso: Fechado
Identificador DOI: 10.1016/j.intimp.2016.07.009
Endereço : https://www.sciencedirect.com/science/article/pii/S1567576916302788
Data do documento: 2016
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