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Type: Artigo
Title: Culture medium of diluted skimmed milk for the production of nisin in batch cultivations
Author: Arauz, Luciana Juncioni de
Jozala, Angela Faustino
Baruque-Ramos, Julia
Mazzola, Priscila Gava
Pessoa, Adalberto
Vessoni Penna, Thereza Christina
Abstract: Nisin is a promising alternative to chemical preservatives for use as a natural biopreservative in foods. This bacteriocin has also potential biomedical applications. Lactic acid bacteria are commonly cultivated in expensive standard complex media. We have evaluated the cell growth and nisin production of Lactococcus lactis in a low-cost natural medium consisting of diluted skimmed milk in a 2-L bioreactor. The assays were performed at 30°C for 56 h, at varying agitation speeds and airflow rates: (1) 200 rpm (no airflow, and airflow at 0.5, 1.0 and 2.0 L/min); (2) 100 rpm (no airflow, and airflow at 0.5 L/min). Nisin activity was evaluated using agar diffusion assays. The highest nisin concentration, 49.88 mg/L (3.3 log AU/mL or 1,995.29 AU/mL), was obtained at 16 h of culture, 200 rpm and no airflow (kL a = 5.29 × 10−3). These results show that a cultivation medium composed of diluted skimmed milk supports cell growth to facilitate nisin biosynthesis
Subject: Bactérias produtoras de ácido láctico
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s13213-011-0278-6
Date Issue: 2012
Appears in Collections:FCM - Artigos e Outros Documentos

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