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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampRodrigues, Eliseu-
dc.contributor.authorunicampMercadante, Adriana Zerlotti-
dc.typeArtigopt_BR
dc.titleIn vivo assessment of the cytotoxic, genotoxic and antigenotoxic potential of maná-cubiu (solanum sessiliflorum dunal) fruitpt_BR
dc.contributor.authorHernandes, Lívia Cristina-
dc.contributor.authorAissa, Alexandre Ferro-
dc.contributor.authorAlmeida, Mara Ribeiro de-
dc.contributor.authorDarin, Joana D'arc Castania-
dc.contributor.authorRodrigues, Eliseu-
dc.contributor.authorBatista, Bruno Lemos-
dc.contributor.authorBarbosa Júnior, Fernando-
dc.contributor.authorMercadante, Adriana Zerlotti-
dc.contributor.authorBianchi, Maria Lourdes Pires-
dc.contributor.authorAntunes, Lusânia Maria Greggi-
dc.subjectEnsaio cometapt_BR
dc.subjectAntioxidantespt_BR
dc.subject.otherlanguageComet assaypt_BR
dc.subject.otherlanguageAntioxidantspt_BR
dc.description.abstractSolanum sessiliflorum Dunal is a native shrub often found in the Amazon Forest. Its fruits, known as maná-cubiu, possess an unusual flavor and are consumed in salads and juices, mainly by the local community of Northern Brazil. Because these fruits are used in traditional medicine as hypoglycemic and hypocholesterolemic agents, it is important to establish whether the consumption of maná-cubiu is safe using in vivo genotoxicity tests. Here, we investigated the genotoxic and antigenotoxic potential of maná-cubiu for doxorubicin (DXR)-induced DNA damage using the micronucleus test and the comet assay in Wistar rats. Moreover, oxidative stress parameters were determined in the heart and liver of the animals by measuring the thiobarbituric acid reactive substances (TBARS), a biomarker of lipid peroxidation, and reduced glutathione (GSH) content. The relative expression of Ptgs2 mRNA in the livers of the animals was also determined. The tests were performed with maná-cubiu pulp (125, 250, 375 or 500 mg/kg body weight — b.w.) by gavage for 14 days, followed by intraperitoneal injection of saline or DXR (16 mg/kg b.w.) immediately after the last gavage, which occurred 24 h before euthanasia. The results showed that maná-cubiu at all tested doses had no cytotoxic effects on bone marrow cells and was not genotoxic to heart or liver cells. In addition, maná-cubiu treatments decreased DXR-induced DNA damage according to the comet assay in heart and liver cells. Reductions in micronucleus frequency in peripheral blood cells occurred at 125, 250 and 375 mg/kg b.w. doses of maná-cubiu, and the TBARS content induced by DXR was also reduced by maná-cubiu. Furthermore, maná-cubiu did not modulate the transcription of the Ptgs2 gene. In conclusion, maná-cubiu pulp fruit was not cytotoxic or genotoxic in Wistar rats, suggesting its safety for human consumption, at least considering genotoxic effects. The antioxidant capacity of maná-cubiu pulp fruit may contribute to the antigenotoxic effects of this fruit at the doses used in this studypt_BR
dc.relation.ispartofFood research internationalpt_BR
dc.relation.ispartofabbreviationFood res. int.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherElsevierpt_BR
dc.date.issued2014-
dc.date.monthofcirculationAug.pt_BR
dc.language.isoengpt_BR
dc.description.volume62pt_BR
dc.description.firstpage121pt_BR
dc.description.lastpage127pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0963-9969pt_BR
dc.identifier.eissn1873-7145pt_BR
dc.identifier.doi10.1016/j.foodres.2014.02.036pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S096399691400146Xpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber471700/2012-6pt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumber#2011/01982-6pt_BR
dc.date.available2020-07-16T19:03:55Z-
dc.date.accessioned2020-07-16T19:03:55Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2020-07-16T19:03:55Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-12T13:36:48Z : No. of bitstreams: 1 000340015100016.pdf: 424410 bytes, checksum: a9f243a6565506307af169b724cbe233 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-07-16T19:03:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/345570-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Ciência de Alimentospt_BR
dc.contributor.unidadeFaculdade de Engenharia de Alimentospt_BR
dc.contributor.unidadeFaculdade de Engenharia de Alimentospt_BR
dc.subject.keywordDoxorubicinpt_BR
dc.subject.keywordMicronucleus testpt_BR
dc.subject.keywordGene expressionpt_BR
dc.identifier.source000340015100016pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0003-4547-6064pt_BR
dc.type.formArtigopt_BR
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