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Type: Artigo
Title: Exosomes in the serum of acute myeloid leukemia patients induce dendritic cell tolerance : implications for immunotherapy
Author: Benites, Bruno Deltreggia
Santos Duarte, Adriana da Silva
Figueiredo Longhini, Ana Leda
Santos, Irene
Alvarez, Marisa Claudia
de Morais Ribeiro, Ligia Nunes
de Paula, Eneida
Olalla Saad, Sara Teresinha
Abstract: Exosomes may represent an interesting antigenic pulse for new forms of anti-tumor immunotherapy. We evaluated exosomes from serum of patients with acute myeloid leukemia (AML) as an antigenic source for dendritic cells (DC) and the effects upon antitumor cytotoxicity, assessed by the percentage of specific lysis of K562 leukemic cells in co-cultures. Surprisingly, incubation of exosomes with DCs decreased lysis of K562, which may correspond to a mechanism of tumor evasion in vivo. However, when immature DCs were pulsed with exosomes purified from K562 culture supernatants, the lysis of target cells was notably enhanced, associated with a substantial increase in the expression of the maturation marker CD83. Thus, the development of vaccines using patients' exosomes would probably add no benefits to the treatment of AML; alternately, exosomes from cultured cells may represent an effective way for maturing DCs into a cytotoxic phenotype, without the immunosuppression observed with patients' exosomes. (C) 2019 Elsevier Ltd. All rights reserved
Subject: Leucemia mielóide aguda
Country: Reino Unido
Editor: Elsevier Science
Rights: fechado
Identifier DOI: 10.1016/j.vaccine.2019.01.079
Date Issue: 2019
Appears in Collections:IB - Artigos e Outros Documentos
FCM - Artigos e Outros Documentos
HEMO - Artigos e Outros Documentos

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