Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328835
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authoremailmlsmello@unicamp.brpt_BR
dc.contributor.authorunicampVeronezi, Giovana Maria Bredapt_BR
dc.contributor.authorunicampFelisbino, Marina Barretopt_BR
dc.contributor.authorunicampGatti, Maria Silvia Viccaript_BR
dc.contributor.authorunicampMello, Maria Luiza Silveirapt_BR
dc.contributor.authorunicampVidal, Benedicto de Campospt_BR
dc.typeArtigopt_BR
dc.titleDna Methylation Changes In Valproic Acid-treated Hela Cells As Assessed By Image Analysis, Immunofluorescence And Vibrational Microspectroscopyen
dc.titleDNA methylation changes in valproic acid-treated HeLa cells as assessed by Image analysis, immunofluorescence and vibrational microspectroscopypt_BR
dc.contributor.authorVeronezi, Giovana M. B.pt_BR
dc.contributor.authorFelisbino, Marina Barretopt_BR
dc.contributor.authorGatti, Maria Silvia V.pt_BR
dc.contributor.authorMello, Maria Luiza S.pt_BR
dc.contributor.authorVidal, Benedicto de Campospt_BR
unicamp.authorVidal, Benedicto de Campos] Univ Campinas Unicamp, Dept Struct & Funct Biol, Sao Paulo, Brazilpt_BR
unicamp.authorVidal, Benedicto de Campos] Univ Campinas Unicamp, Inst Biol, Sao Paulo, Brazilpt_BR
unicamp.author[Gatti, Maria Silvia V.] Univ Campinas Unicamp, Dept Genet Evolut & Bioagents, Inst Biol, Sao Paulo, Brazilpt_BR
unicamp.author.external[Veronezi, Giovana M. B.pt_BR
unicamp.author.externalFelisbino, Marina Barretopt_BR
unicamp.author.externalMello, Maria Luiza S.pt_BR
unicamp.author.external[Veronezi, Giovana M. B.pt_BR
unicamp.author.externalFelisbino, Marina Barretopt_BR
unicamp.author.externalMello, Maria Luiza S.pt_BR
dc.subjectMetilação de DNApt_BR
dc.subjectCélulas HeLapt_BR
dc.subjectÁcido valproicopt_BR
dc.subjectCromatinapt_BR
dc.subjectAnálise de imagempt_BR
dc.subjectFourier, Espectroscopia de infravermelho por transformada dept_BR
dc.subject.otherlanguageDNA methylationpt_BR
dc.subject.otherlanguageHeLa cellspt_BR
dc.subject.otherlanguageValproic acidpt_BR
dc.subject.otherlanguageChromatinpt_BR
dc.subject.otherlanguageImage analysispt_BR
dc.subject.otherlanguageFourier transform infrared spectroscopypt_BR
dc.description.abstractValproic acid (VPA), a well-known histone deacetylase inhibitor, has been reported to affect the DNA methylation status in addition to inducing histone hyperacetylation in several cell types. In HeLa cells, VPA promotes histone acetylation and chromatin remodeling. However, DNA demethylation was not checked in this cell model for standing effects longer than those provided by histone acetylation, which is a rapid and transient phenomenon. Demonstration of VPA-induced DNA demethylation in HeLa cells would contribute to understanding the effect of VPA on an aggressive tumor cell line. In the present work, DNA demethylation in VPA-treated HeLa cells was assessed by image analysis of chromatin texture, the abundance of 5-methylcytosine (5mC) immunofluorescence signals and Fourier transform-infrared (FT-IR) microspectroscopy centered on spectral regions related to the vibration of-(CH3 groups. Image analysis indicated that increased chromatin unpacking promoted by a 4-htreatment with 1.0 mM VPA persisted for 24 h in the absence of the drug, suggesting the occurrence of DNA demethylation that was confirmed by decreased 5mC immunofluorescence signals. FT-IR spectra of DNA samples from 1 mM or 20 mM VPA-treated cells subjected to a peak fitting analysis of the spectral window for-CH3 stretching vibrations showed decreased vibrations and energy of these groups as a function of the decreased abundance of 5mC induced by increased VPA concentrations. Only the 20 mM-VPA treatment caused an increase in the ratio of-CH3 bending vibrations evaluated at 1375 cm(-1) in relation to inplane vibrations of overall cytosines evaluated at 1492 cm(-1). CH3 stretching vibrations showed to be more sensitive than-CH3 bending vibrations, as detected with FT-IR microspectroscopy, for studies aiming to associate vibrational spectroscopy and changes in DNA 5mC abundance.en
dc.description.abstractValproic acid (VPA), a well-known histone deacetylase inhibitor, has been reported to affect the DNA methylation status in addition to inducing histone hyperacetylation in several cell types. In HeLa cells, VPA promotes histone acetylation and chromatin remodeling. However, DNA demethylation was not checked in this cell model for standing effects longer than those provided by histone acetylation, which is a rapid and transient phenomenon. Demonstration of VPA-induced DNA demethylation in HeLa cells would contribute to understanding the effect of VPA on an aggressive tumor cell line. In the present work, DNA demethylation in VPA-treated HeLa cells was assessed by image analysis of chromatin texture, the abundance of 5-methylcytosine (5mC) immunofluorescence signals and Fourier transform-infrared (FT-IR) microspectroscopy centered on spectral regions related to the vibration of-(CH3 groups. Image analysis indicated that increased chromatin unpacking promoted by a 4-htreatment with 1.0 mM VPA persisted for 24 h in the absence of the drug, suggesting the occurrence of DNA demethylation that was confirmed by decreased 5mC immunofluorescence signals. FT-IR spectra of DNA samples from 1 mM or 20 mM VPA-treated cells subjected to a peak fitting analysis of the spectral window for-CH3 stretching vibrations showed decreased vibrations and energy of these groups as a function of the decreased abundance of 5mC induced by increased VPA concentrations. Only the 20 mM-VPA treatment caused an increase in the ratio of-CH3 bending vibrations evaluated at 1375 cm(-1) in relation to inplane vibrations of overall cytosines evaluated at 1492 cm(-1). CH3 stretching vibrations showed to be more sensitive than-CH3 bending vibrations, as detected with FT-IR microspectroscopy, for studies aiming to associate vibrational spectroscopy and changes in DNA 5mC abundance.pt
dc.relation.ispartofPlos onept_BR
dc.relation.ispartofabbreviationPLoS onept_BR
dc.publisher.citySan Francisco, CApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherPublic Library of Sciencept_BR
dc.date.issued2017pt_BR
dc.date.monthofcirculationJan.pt_BR
dc.identifier.citationPlos One. Public Library Science, v. 12, p. , 2017.pt_BR
dc.language.isoengpt_BR
dc.description.volume12pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage1pt_BR
dc.description.lastpage13pt_BR
dc.rightsabertopt_BR
dc.sourceWOSpt_BR
dc.sourceWOSpt_br
dc.identifier.eissn1932-6203pt_BR
dc.identifier.wosidWOS:000396129000092pt_BR
dc.identifier.doi10.1371/journal.pone.0170740pt_BR
dc.identifier.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170740pt_BR
dc.description.sponsorshipFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorshipFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorshipCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsordocumentnumber2007/58251-8pt_BR
dc.description.sponsordocumentnumber2014/23842-0pt_BR
dc.description.sponsordocumentnumber304668/2014-1pt_BR
dc.date.available2017-11-13T13:44:41Z-
dc.date.accessioned2017-11-13T13:44:41Z-
dc.description.provenanceMade available in DSpace on 2017-11-13T13:44:41Z (GMT). No. of bitstreams: 1 000396129000092.pdf: 2235998 bytes, checksum: a3c54d38f6e33b8caf0bf164deaf1cae (MD5) Previous issue date: 2017en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328835-
dc.contributor.departmentDepartamento de Genética, Evolução e Bioagentespt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.description.abstractalternative-
dc.identifier.source396129000092-
dc.creator.orcid0000-0003-3614-5503pt_BR
dc.creator.orcid0000-0003-1629-5619pt_BR
dc.creator.orcid0000-0003-0285-1653pt_BR
dc.type.formArtigo de pesquisa-
Appears in Collections:IB - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000396129000092.pdf2.18 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.