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Type: Artigo
Title: Protein Expression In Human Cumulus Cells As An Indicator Of Blastocyst Formation And Pregnancy Success
Author: Almeida Ferreira Braga
Daniela Paes; Setti
Amanda Souza; Lo Turco
Edson Guimares; Cordeiro
Fernanda Bertuccez; Cabral
Elaine Cristina; Cortezzi
Sylvia Sanches; Ono
Erika; Savio Figueira
Rita Cassia; Eberlin
Marcos Nogueira; Borges
Abstract: The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.
Subject: Cumulus Cell
Mass Spectrometry
Editor: Springer/Plenum Publishers
New York
Citation: Journal Of Assisted Reproduction And Genetics. Springer/plenum Publishers, v. 33, p. 1571 - 1583, 2016.
Rights: fechado
Identifier DOI: 10.1007/s10815-016-0800-7
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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