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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authoremailmarcosjs@unicamp.brpt_BR
dc.typeArtigopt_BR
dc.titleEsi-ms Fingerprinting Of Residues Of Green Propolis, And Evaluation Of Their Antioxidant And Antimicrobial Activitiesen
dc.contributor.authorCorreapt_BR
dc.contributor.authorWallace Ribeiro; Lopezpt_BR
dc.contributor.authorBegona Gimenez-Cassina; do Pradopt_BR
dc.contributor.authorSheila Cristina; da Silva Cunhapt_BR
dc.contributor.authorIldenize Barbosa; Helena Frankland Sawayapt_BR
dc.contributor.authorAlexandra Christine; Salvadorpt_BR
dc.contributor.authorMarcos Josept_BR
unicamp.authorSalvador, Marcos Jose] State Univ Campinas UNICAMP, Inst Biol, PPG BTPB & PPG BV, Dept Plant Biol, Campinas, Brazilpt_BR
unicamp.author[da Silva Cunha, Ildenize Barbosa] IQ UNICAMP, Thomson Mass Spectrometry Lab, Campinas, Brazilpt_BR
unicamp.author.external[Correa, Wallace Ribeiropt_BR
unicamp.author.externalLopez, Begona Gimenez-Cassinapt_BR
unicamp.author.externalda Silva Cunha, Ildenize Barbosapt_BR
unicamp.author.externalHelena Frankland Sawaya, Alexandra Christinept_BR
unicamp.author.external[Correa, Wallace Ribeiropt_BR
unicamp.author.externaldo Prado, Sheila Cristina] Fed Inst Educ Sci & Technol, South Of Minas Gerais, Inconfidentes, Brazilpt_BR
dc.subjectElectrospray Ionization Mass Spectrometry Fingerprintingen
dc.subjectPropolisen
dc.subjectAntioxidant Activityen
dc.subjectAntimicrobial Activityen
dc.description.abstractIn this study, we evaluated the composition of the residues of subsequent extractions of raw propolis compared to that of the first extraction (EEP) in order to determine the appropriate number of extractions that could be used. Three samples of green propolis, two of them from small apiaries and another from a larger apiary (all with similar composition) were analyzed. The chemical composition of the residues and the crude propolis was evaluated by direct insertion electrospray ionization mass spectrometry in the negative ion mode (ESI(-)-MS) using a TQD Acquity mass spectrometer. The antioxidant activity was assessed by DPPH and ORAC assays, and the antimicrobial activity was studied as well. A comparison of the ESI(-)-MS fingerprints showed that bioactive substances still remain in the residues after one or two extractions. Artepillin C was found to be present in green propolis extract (EEP) and also in the residues; however it was present in lower concentrations. Nevertheless, another important substance, dicaffeoylquinic acid, was only found in the propolis extract and in the first two residues. In the DPPH and ORAC assays, a sequential loss of antioxidant activity was observed. The antimicrobial activity of EEP and extract of the first two residues was investigated in vitro against gram-positive and gram-negative bacteria and EEP, and the first extract presented inhibition values of 1mgml(-1) or less. Two sequential extractions of raw green propolis are appropriate to avoid the loss of important chemical components in the residue.en
dc.relation.ispartofJournal of Apicultural Researchpt_BR
dc.publisherTaylor & Francis Ltdpt_BR
dc.publisherAbingdonpt_BR
dc.date.issued2016pt_BR
dc.identifier.citationJournal Of Apicultural Research. Taylor & Francis Ltd, v. 55, p. 1 - 7, 2016.pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume55pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage1pt_BR
dc.description.lastpage7pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0021-8839pt_BR
dc.identifier.eissn2078-6913pt_BR
dc.identifier.wosidWOS:000382966500001pt_BR
dc.identifier.doi10.1080/00218839.2016.1196014pt_BR
dc.identifier.urlhttp://www.tandfonline.com/doi/abs/10.1080/00218839.2016.1196014pt_BR
dc.description.sponsorshipCAPESpt_BR
dc.description.sponsorshipFAPESP [2013/3020313-3, 2008/58035-6]pt_BR
dc.description.sponsorshipIFSULDEMINASpt_BR
dc.description.sponsorshipFAEPEXpt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.date.available2017-11-13T11:32:06Z-
dc.date.accessioned2017-11-13T11:32:06Z-
dc.description.provenanceMade available in DSpace on 2017-11-13T11:32:06Z (GMT). No. of bitstreams: 1 000382966500001.pdf: 701291 bytes, checksum: c3c79a57d5bf359c82117e87229229c4 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/326020-
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