Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/320194
Type: Artigo
Title: Multimodal and non-linear optical microscopy applications in reproductive biology
Author: Adur, J.
Barbosa, G. O.
Pelegati, V. B.
Baratti, M. O.
Cesar, C. L.
Casco, V. H.
Carvalho, H. F.
Abstract: A plethora of optical techniques is currently available to obtain non-destructive, contactless, real time information with subcellular spatial resolution to observe cell processes. Each technique has its own unique features for imaging and for obtaining certain biological information. However none of the available techniques can be of universal use. For a comprehensive investigation of biological specimens and events, one needs to use a combination of bioimaging methods, often at the same time. Some modern confocal/multiphoton microscopes provide simultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Some of them can also easily be adapted for harmonic generation imaging, and to permit cell manipulation technique. In this work we present a multimodal optical workstation that extends a commercially available confocal microscope to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercial confocal microscope by exploiting all the flexibility offered by the manufacturer. The various capabilities of this workstation as applied directly to reproductive biology are discussed. (C) 2016 Wiley Periodicals, Inc.
A plethora of optical techniques is currently available to obtain non-destructive, contactless, real time information with subcellular spatial resolution to observe cell processes. Each technique has its own unique features for imaging and for obtaining certain biological information. However none of the available techniques can be of universal use. For a comprehensive investigation of biological specimens and events, one needs to use a combination of bioimaging methods, often at the same time. Some modern confocal/multiphoton microscopes provide simultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Some of them can also easily be adapted for harmonic generation imaging, and to permit cell manipulation technique. In this work we present a multimodal optical workstation that extends a commercially available confocal microscope to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercial confocal microscope by exploiting all the flexibility offered by the manufacturer. The various capabilities of this workstation as applied directly to reproductive biology are discussed.
Subject: Microscopia confocal
Microscopia confocal multifóton
Country: Estados Unidos
Editor: Wiley-Blackwell
Citation: Microscopy Research And Technique. WILEY-BLACKWELL, n. 79, n. 7, p. 567 - 582.
Rights: fechado
Identifier DOI: 10.1002/jemt.22684
Address: https://onlinelibrary.wiley.com/doi/full/10.1002/jemt.22684
Date Issue: 2016
Appears in Collections:IFGW - Artigos e Outros Documentos
IB - Artigos e Outros Documentos

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