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Type: Artigo de periódico
Title: Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography for fatty acid profiling of cell wall phospholipids
Author: Bogusz, Stanislau, Jr.
Hantao, Leandro Wang
Gonzaga Neves Braga, Soraia Cristina
Rodrigues de Matos Franca, Valtenice de Cassia
da Costa, Marcelo Fernandes
Hamer, Russell David
Ventura, Dora Fix
Augusto, Fabio
Abstract: The combination of solid-phase microextraction (SPME) with comprehensive two-dimensional gas chromatography is evaluated here for fatty acid (FA) profiling of the glycerophospholipid fraction from human buccal mucosal cells. A base-catalyzed derivatization reaction selective for polar lipids such as glycerophospholipid was adopted. SPME is compared to a miniaturized liquidliquid extraction procedure for the isolation of FA methyl esters produced in the derivatization step. The limits of detection and limits of quantitation were calculated for each sample preparation method. Because of its lower values of limits of detection and quantitation, SPME was adopted. The extracted analytes were separated, detected, and quantified by comprehensive two-dimensional gas chromatography with flame ionization detection (FID). The combination of SPME and comprehensive two-dimensional gas chromatography with FID, using a selective derivatization reaction in the preliminary steps, proved to be a simple and fast procedure for FA profiling, and was successfully applied to the analysis of adult human buccal mucosal cells.
Subject: Comprehensive two-dimensional gas chromatography
Fatty acid methyl ester
Human buccal mucosal cells
Selective methanolysis
Solid-phase microextraction
Editor: Wiley-Blackwell
Citation: Journal of Separation Science. Wiley-Blackwell, v.35, n.18, p.2438-2444, 2012
Rights: fechado
Identifier DOI: 10.1002/jssc.201200256
Date Issue: 2012
Appears in Collections:IQ - Artigos e Outros Documentos

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