Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/244215
Type: Artigo de periódico
Title: Direct Protocol For Ambient Mass Spectrometry Imaging On Agar Culture
Author: Angolini
Celio Fernando F.; Vendramini
Pedro Henrique; Araujo
Francisca D. S.; Araujo
Welington L.; Augusti
Rodinei; Eberlin
Marcos N.; de Oliveira
Luciana Gonzaga
Abstract: Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EAST) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.
Subject: Desorption Electrospray-ionization
Siderophore Production
Iron Transport
Resistance
Bacteria
Exchange
Shuttle
Growth
Fungi
Country: WASHINGTON
Editor: AMER CHEMICAL SOC
Citation: Direct Protocol For Ambient Mass Spectrometry Imaging On Agar Culture. Amer Chemical Soc, v. 87, p. 6925-6930 Jul-2015.
Rights: fechado
Identifier DOI: 10.1021/acs.analchem.5b01538
Address: http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b01538
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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