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http://repositorio.unicamp.br/jspui/handle/REPOSIP/244021
Type: | Artigo |
Title: | Low Mir-143/mir-145 cluster levels induce activin a overexpression in oral squamous cell carcinomas, which contributes to poor prognosis |
Author: | Bufalino, Andreia Cervigne, Nilva K. de Oliveira, Carine Ervolino Fonseca, Felipe Paiva Rodrigues, Priscila Campioni Soares Macedo, Carolina Carneiro Sobral, Lays Martin Miguel, Marcia Costa Lopes, Marcio Ajudarte Paes Leme, Adriana Franco Lambe |
Abstract: | Deregulated expression of activin A is reported in several tumors, but its biological functions in oral squamous cell carcinoma (OSCC) are unknown. Here, we investigate whether activin A can play a causal role in OSCCs. Activin A expression was assessed by qPCR and immunohistochemistry in OSCC tissues. Low activin A-expressing cells were treated with recombinant activin A and assessed for apoptosis, proliferation, adhesion, migration, invasion and epithelial-mesenchymal transition (EMT). Those phenotypes were also evaluated in high activin A-expressing cells treated with follistatin (an activin A antagonist) or stably expressing shRNA targeting activin A. Transfections of microRNA mimics were performed to determine whether the overexpression of activin A is regulated by miR-143/miR-145 cluster. Activin A was overexpressed in OSCCs in comparison with normal oral mucosa, and high activin A levels were significantly associated with lymph node metastasis, tumor differentiation and poor survival. High activin A levels promoted multiple properties associated with malignant transformation, including decreased apoptosis and increased proliferation, migration, invasion and EMT. Both miR-143 and miR-145 were markedly downregulated in OSCC cell lines and in clinical specimens, and inversely correlated to activin A levels. Forced expression of miR-143 and miR-145 in OSCC cells significantly decreased the expression of activin A. Overexpression of activin A in OSCCs, which is controlled by downregulation of miR-143/miR-145 cluster, regulates apoptosis, proliferation and invasiveness, and it is clinically correlated with lymph node metastasis and poor survival. Deregulated expression of activin A is reported in several tumors, but its biological functions in oral squamous cell carcinoma (OSCC) are unknown. Here, we investigate whether activin A can play a causal role in OSCCs. Activin A expression was assessed b |
Subject: | Apoptose Metástase Neoplasias da próstata |
Country: | Estados Unidos |
Editor: | Public Library of Science |
Citation: | Low Mir-143/mir-145 Cluster Levels Induce Activin A Overexpression In Oral Squamous Cell Carcinomas, Which Contributes To Poor Prognosis. Public Library Science, v. 10, p. AUG-2015. |
Rights: | aberto Aberto |
Identifier DOI: | 10.1371/journal.pone.0136599 |
Address: | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136599 |
Date Issue: | 2015 |
Appears in Collections: | FOP - Artigos e Outros Documentos |
Files in This Item:
File | Size | Format | |
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000360299100108.pdf | 4.44 MB | Adobe PDF | View/Open |
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