Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/244021
Type: Artigo
Title: Low Mir-143/mir-145 cluster levels induce activin a overexpression in oral squamous cell carcinomas, which contributes to poor prognosis
Author: Bufalino, Andreia
Cervigne, Nilva K.
de Oliveira, Carine Ervolino
Fonseca, Felipe Paiva
Rodrigues, Priscila Campioni
Soares Macedo, Carolina Carneiro
Sobral, Lays Martin
Miguel, Marcia Costa
Lopes, Marcio Ajudarte
Paes Leme, Adriana Franco
Lambe
Abstract: Deregulated expression of activin A is reported in several tumors, but its biological functions in oral squamous cell carcinoma (OSCC) are unknown. Here, we investigate whether activin A can play a causal role in OSCCs. Activin A expression was assessed by qPCR and immunohistochemistry in OSCC tissues. Low activin A-expressing cells were treated with recombinant activin A and assessed for apoptosis, proliferation, adhesion, migration, invasion and epithelial-mesenchymal transition (EMT). Those phenotypes were also evaluated in high activin A-expressing cells treated with follistatin (an activin A antagonist) or stably expressing shRNA targeting activin A. Transfections of microRNA mimics were performed to determine whether the overexpression of activin A is regulated by miR-143/miR-145 cluster. Activin A was overexpressed in OSCCs in comparison with normal oral mucosa, and high activin A levels were significantly associated with lymph node metastasis, tumor differentiation and poor survival. High activin A levels promoted multiple properties associated with malignant transformation, including decreased apoptosis and increased proliferation, migration, invasion and EMT. Both miR-143 and miR-145 were markedly downregulated in OSCC cell lines and in clinical specimens, and inversely correlated to activin A levels. Forced expression of miR-143 and miR-145 in OSCC cells significantly decreased the expression of activin A. Overexpression of activin A in OSCCs, which is controlled by downregulation of miR-143/miR-145 cluster, regulates apoptosis, proliferation and invasiveness, and it is clinically correlated with lymph node metastasis and poor survival.
Deregulated expression of activin A is reported in several tumors, but its biological functions in oral squamous cell carcinoma (OSCC) are unknown. Here, we investigate whether activin A can play a causal role in OSCCs. Activin A expression was assessed b
Subject: Apoptose
Metástase
Neoplasias da próstata
Country: Estados Unidos
Editor: Public Library of Science
Citation: Low Mir-143/mir-145 Cluster Levels Induce Activin A Overexpression In Oral Squamous Cell Carcinomas, Which Contributes To Poor Prognosis. Public Library Science, v. 10, p. AUG-2015.
Rights: aberto
Aberto
Identifier DOI: 10.1371/journal.pone.0136599
Address: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136599
Date Issue: 2015
Appears in Collections:FOP - Artigos e Outros Documentos

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