Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/243953
Type: Artigo
Title: Taurine supplementation ameliorates glucose homeostasis, prevents insulin and glucagon hypersecretion, and controls Beta, Alpha, and Delta-cell masses in genetic obese mice
Author: Santos-Silva, Junia C.
Ribeiro, Rosane Aparecida
Vettorazzi, Jean F.
Irles, Esperanza
Rickli, Sarah
Borck, Patrícia C.
Porciuncula, Patrícia M.
Quesada, Ivan
Nadal, Angel
Boschero, Antonio C.
Carneiro, Everardo M.
Abstract: Taurine (Tau) regulates beta-cell function and glucose homeostasis under normal and diabetic conditions. Here, we assessed the effects of Tau supplementation upon glucose homeostasis and the morphophysiology of endocrine pancreas, in leptin-deficient obese (ob) mice. From weaning until 90-day-old, C57Bl/6 and ob mice received, or not, 5 % Tau in drinking water (C, CT, ob and obT). Obese mice were hyperglycemic, glucose intolerant, insulin resistant, and exhibited higher hepatic glucose output. Tau supplementation did not prevent obesity, but ameliorated glucose homeostasis in obT. Islets from ob mice presented a higher glucose-induced intracellular Ca2+ influx, NAD(P)H production and insulin release. Furthermore, alpha-cells from ob islets displayed a higher oscillatory Ca2+ profile at low glucose concentrations, in association with glucagon hypersecretion. In Tau-supplemented ob mice, insulin and glucagon secretion was attenuated, while Ca2+ influx tended to be normalized in beta-cells and Ca2+ oscillations were increased in alpha-cells. Tau normalized the inhibitory action of somatostatin (SST) upon insulin release in the obT group. In these islets, expression of the glucagon, GLUT-2 and TRPM5 genes was also restored. Tau also enhanced MafA, Ngn3 and NeuroD mRNA levels in obT islets. Morphometric analysis demonstrated that the hypertrophy of ob islets tends to be normalized by Tau with reductions in islet and beta-cell masses, but enhanced delta-cell mass in obT. Our results indicate that Tau improves glucose homeostasis, regulating beta-, alpha-, and delta-cell morphophysiology in ob mice, indicating that Tau may be a potential therapeutic tool for the preservation of endocrine pancreatic function in obesity and diabetes.
Taurine (Tau) regulates beta-cell function and glucose homeostasis under normal and diabetic conditions. Here, we assessed the effects of Tau supplementation upon glucose homeostasis and the morphophysiology of endocrine pancreas, in leptin-deficient obese (ob) mice. From weaning until 90-day-old, C57Bl/6 and ob mice received, or not, 5 % Tau in drinking water (C, CT, ob and obT). Obese mice were hyperglycemic, glucose intolerant, insulin resistant, and exhibited higher hepatic glucose output. Tau supplementation did not prevent obesity, but ameliorated glucose homeostasis in obT. Islets from ob mice presented a higher glucose-induced intracellular Ca2+ influx, NAD(P)H production and insulin release. Furthermore, alpha-cells from ob islets displayed a higher oscillatory Ca2+ profile at low glucose concentrations, in association with glucagon hypersecretion. In Tau-supplemented ob mice, insulin and glucagon secretion was attenuated, while Ca2+ influx tended to be normalized in beta-cells and Ca2+ oscillations were increased in alpha-cells. Tau normalized the inhibitory action of somatostatin (SST) upon insulin release in the obT group. In these islets, expression of the glucagon, GLUT-2 and TRPM5 genes was also restored. Tau also enhanced MafA, Ngn3 and NeuroD mRNA levels in obT islets. Morphometric analysis demonstrated that the hypertrophy of ob islets tends to be normalized by Tau with reductions in islet and beta-cell masses, but enhanced delta-cell mass in obT. Our results indicate that Tau improves glucose homeostasis, regulating beta-, alpha-, and delta-cell morphophysiology in ob mice, indicating that Tau may be a potential therapeutic tool for the preservation of endocrine pancreatic function in obesity and diabetes
Subject: Glucagon
Insulina - Secreção
Obesidade
Somatostatina
Taurina
Homeostase
Country: Austria
Editor: Springer
Citation: Taurine Supplementation Ameliorates Glucose Homeostasis, Prevents Insulin And Glucagon Hypersecretion, And Controls Beta, Alpha, And Delta-cell Masses In Genetic Obese Mice. Springer Wien, v. 47, p. 1533-1548 AUG-2015.
Rights: fechado
Identifier DOI: 10.1007/s00726-015-1988-z
Address: http://link.springer.com/article/10.1007%2Fs00726-015-1988-z
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos

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