Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242325
Type: Artigo de periódico
Title: Revealing Praziquantel Molecular Targets Using Mass Spectrometry Imaging: An Expeditious Approach Applied To Schistosoma Mansoni
Author: Ferreira
Monica Siqueira; de Oliveira
Rosimeire Nunes; de Oliveira
Diogo Noin; Esteves
Cibele Zanardi; Allegretti
Silmara Marques; Catharino
Rodrigo Ramos
Abstract: Finding specific molecular targets and the mechanism of action of praziquantel in the treatment of schistosomiasis remains a challenging task. Our efforts were focused on obtaining further information on worm composition before and after exposure to praziquantel in the treatment of schistosomiasis to elucidate the potential sites of action of this drug. Evidence indicates that the lipid bilayer is changed by treatment with praziquantel. Following this rationale, we employed a mass spectrometry imaging-based approach that helped to characterise lipids in specific locations, which are directly involved in the biochemical pathways of the BH strain of Schistosoma mansoni, as well as differentiating the molecular response that each worm sex presents in vivo. Our findings demonstrated significant differences between the chemical markers found in adult worms before and after praziquantel exposure, especially in phospholipids, which were predominantly identified as chemical markers in all samples. Results also indicate that distinct molecular pathways in both male and female worms could be differentially affected by praziquantel treatment. These data shine new light on the mechanism of action of praziquantel, taking a further step towards its full understanding. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Subject: Tumor-necrosis-factor
Sphingomyelin Cycle
Protein-kinase
Factor-alpha
Maldi-msi
Ceramide
Lipids
Mechanisms
Expression
Infection
Country: OXFORD
Editor: ELSEVIER SCI LTD
Citation: Revealing Praziquantel Molecular Targets Using Mass Spectrometry Imaging: An Expeditious Approach Applied To Schistosoma Mansoni. Elsevier Sci Ltd, v. 45, p. 385-391 MAY-2015.
Rights: embargo
Identifier DOI: 10.1016/j.ijpara.2014.12.008
Address: http://www.sciencedirect.com/science/article/pii/S0020751915000491
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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