Use este identificador para citar ou linkar para este item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242229
Tipo: Artigo
Título: A low-protein diet during pregnancy prevents modifications in intercellular communication proteins in rat islets
Autor(es): Marçal-Pessoa, Ana Flávia
Bassi-Branco, Carmen Lucia
Salvatierra, Cristiana dos Santos Barbosa
Stoppiglia, Luiz Fabrizio
Ignacio-Souza, Letícia Martins
Reis, Sílvia Regina de Lima
Veloso, Roberto Vilela
Reis, Marise Auxiliadora de Barros
Carneiro, Everardo Magalhães
Boschero, Antonio Carlos
Arantes, Vanessa Cristina
Latorraca, Márcia Queiroz
Resumo: Background: Gap junctions between beta-cells participate in the precise regulation of insulin secretion. Adherens junctions and their associated proteins are required for the formation, function and structural maintenance of gap junctions. Increases in the number of the gap junctions between beta-cells and enhanced glucose-stimulated insulin secretion are observed during pregnancy. In contrast, protein restriction produces structural and functional alterations that result in poor insulin secretion in response to glucose. We investigated whether protein restriction during pregnancy affects the expression of mRNA and proteins involved in gap and adherens junctions in pancreatic islets. An isoenergetic low-protein diet (6% protein) was fed to non-pregnant or pregnant rats from day 1-15 of pregnancy, and rats fed an isocaloric normal-protein diet (17% protein) were used as controls. Results: The low-protein diet reduced the levels of connexin 36 and beta-catenin protein in pancreatic islets. In rats fed the control diet, pregnancy increased the levels of phospho-[Ser279/282]-connexin 43, and it decreased the levels of connexin 36, beta-catenin and beta-actin mRNA as well as the levels of connexin 36 and beta-catenin protein in islets. The low-protein diet during pregnancy did not alter these mRNA and protein levels, but avoided the increase of levels of phospho-[Ser279/282]-connexin 43 in islets. Insulin secretion in response to 8.3 mmol/L glucose was higher in pregnant rats than in non-pregnant rats, independently of the nutritional status. Conclusion: Short-term protein restriction during pregnancy prevented the Cx43 phosphorylation, but this event did not interfer in the insulin secretion.
Gap junctions between beta-cells participate in the precise regulation of insulin secretion. Adherens junctions and their associated proteins are required for the formation, function and structural maintenance of gap junctions. Increases in the number of the gap junctions between beta-cells and enhanced glucose-stimulated insulin secretion are observed during pregnancy. In contrast, protein restriction produces structural and functional alterations that result in poor insulin secretion in response to glucose. We investigated whether protein restriction during pregnancy affects the expression of mRNA and proteins involved in gap and adherens junctions in pancreatic islets. An isoenergetic low-protein diet (6% protein) was fed to non-pregnant or pregnant rats from day 1-15 of pregnancy, and rats fed an isocaloric normal-protein diet (17% protein) were used as controls. The low-protein diet reduced the levels of connexin 36 and beta-catenin protein in pancreatic islets. In rats fed the control diet, pregnancy increased the levels of phospho-[Ser279/282]-connexin 43, and it decreased the levels of connexin 36, beta-catenin and beta-actin mRNA as well as the levels of connexin 36 and beta-catenin protein in islets. The low-protein diet during pregnancy did not alter these mRNA and protein levels, but avoided the increase of levels of phospho-[Ser279/282]-connexin 43 in islets. Insulin secretion in response to 8.3 mmol/L glucose was higher in pregnant rats than in non-pregnant rats, independently of the nutritional status. Short-term protein restriction during pregnancy prevented the Cx43 phosphorylation, but this event did not interfer in the insulin secretion
Palavras-chave: Conexina 43
Ilhotas pancreáticas
Prenhez
Dieta com restrição de proteínas
beta Catenina
Conexinas
País: Chile
Editor: Sociedad de Biologia de Chile
Citação: A Low-protein Diet During Pregnancy Prevents Modifications In Intercellular Communication Proteins In Rat Islets. Soc Biolgia Chile, v. 48, p. Jan-2015.
Tipo de Acesso: aberto
Identificador DOI: 10.1186/0717-6287-48-3
Endereço : http://biolres.biomedcentral.com/articles/10.1186/0717-6287-48-3
Data do documento: 2015
Aparece nas coleções:IB - Artigos e Outros Documentos

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