Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242174
Type: Artigo de periódico
Title: The Influence Of Different Referencing Methods On The Accuracy Of C-13 Value Measurement Of Ethanol Fuel By Gas Chromatography/combustion/isotope Ratio Mass Spectrometry
Author: Neves
Laura A.; Rodrigues
Janaina M.; Daroda
Romeu J.; Silva
Paulo R. M.; Ferreira
Alexandre A.; Aranda
Donato A. G.; Eberlin
Marcos N.; Fasciotti
Maira
Abstract: RationaleBrazil is the largest producer of sugar cane bioethanol in the world. Isotope ratio mass spectrometry (IRMS) is the technique of choice to certify the origin/raw materials for ethanol production, but the lack of certified reference materials (CRMs) for accurate measurements of C-13 values traceable to Vienna Pee Dee Belemnite (VPDB), the international zero point for C-13/C-12 measurements, certified and compatible with gas chromatography (GC)/IRMS instruments may compromise the accuracy of C-13 determinations. MethodsWe evaluated the influence of methods for the calibration and normalization of raw C-13 values of ethanol samples. Samples were analyzed by GC/C/IRMS using two different GC columns. Different substances were used as isotopic standards for the working gas calibration. The C-13 values obtained with the three methods of normalization were statistically compared with those obtained with elemental analyzer (EA)/IRMS, since the C-13 results obtained using EA are traceable to VPDB via the NBS 22 reference material. ResultsIt was observed that both the isotopic reference material for CO2 calibration and the GC column have a major effect on the C-13 measurements, leading to a bias of almost 2-3 in the C-13 values. All three methods of normalization were equivalent in performance, enabling an improvement in the GC/C/IRMS accuracy, compared with the EA/IRMS reference values for the samples. ConclusionsAll the methods of CO2 calibration, chromatography and normalization presented in this work demonstrated several sources of traceability and accuracy loss for the determination of C-13 values in ethanol fuel samples by GC/C/IRMS. This work has also shown the importance of using proper CRMs traceable to VPBD that should be compatible and certified using GC/C/IRMS, ideally in a wide range of C-13 values. This is important not only for bioethanol fuel samples, but also for many analytes commonly analyzed by IRMS. Copyright (c) 2015 John Wiley & Sons, Ltd.
Subject: Stable-isotope-ratio
Acetic-acid
Chromatography
C-13/c-12
Liquid
Photosynthesis
Discrimination
Authenticity
Challenges
Guidelines
Country: HOBOKEN
Editor: WILEY-BLACKWELL
Citation: The Influence Of Different Referencing Methods On The Accuracy Of C-13 Value Measurement Of Ethanol Fuel By Gas Chromatography/combustion/isotope Ratio Mass Spectrometry. Wiley-blackwell, v. 29, p. 1938-1946 Nov-2015.
Rights: fechado
Identifier DOI: 10.1002/rcm.7298
Address: http://onlinelibrary.wiley.com/doi/10.1002/rcm.7298/full
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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