Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242123
Type: Artigo de periódico
Title: Use Of Multivariate Statistical Techniques To Optimize The Separation Of 17 Capsinoids By Ultra Performance Liquid Chromatography Using Different Columns
Author: Coutinho
Janclei P.; Barbero
Gerardo F.; Avellan
Oreto F.; Garces-Claver
A.; Godoy
Helena T.; Palma
Miguel; Barroso
Carmelo G.
Abstract: In this work a multivariate statistical tool (Derringer and Suich optimization) was proposed for the separation of seventeen capsinoids (natural and synthetic) using the UHPLC-DAD chromatography. Capsinoids were analyzed at 280 nm. The variables optimized were the mobile phase (water (0.1% acetic acid as solvent A) and acetonitrile (0.1% as solvent B)), gradient time and flow rate. Two columns with different length (50 and 100 mm) were used for the chromatographic separation. The two columns used properly separated the seventeen capsinoids, however the 100 mm column length showed a better chromatographic separation with a shorter run time and smaller peak widths. These results provided better values of limit of detection and quantification for the 100 mm column length. The better conditions of separation with the 100 mm column length were established with: initial mobile phase with 41.8% of solvent B; 3.96 min of linear gradient time to reach 100% of solvent B; flow rate of 0.679 mL min(-1). A validation of the method has been done with excellent values of repeatability (RSD <1.92) and intermediate precision (RSD <3.92). The developed method has been applied to real samples. Capsiate has been identified and quantified in some varieties of peppers. (C) 2014 Elsevier B.V. All rights reserved.
Subject: Ch-19 Sweet
Capsaicinoids
Pepper
Extraction
Cultivar
Capsiate
Humans
Rats
Dad
Country: AMSTERDAM
Editor: ELSEVIER SCIENCE BV
Citation: Use Of Multivariate Statistical Techniques To Optimize The Separation Of 17 Capsinoids By Ultra Performance Liquid Chromatography Using Different Columns. Elsevier Science Bv, v. 134, p. 256-263 MAR-2015.
Rights: embargo
Identifier DOI: 10.1016/j.talanta.2014.11.004
Address: http://www.sciencedirect.com/science/article/pii/S0039914014008960
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
wos_000349723600033.pdf584.92 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.