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Type: Artigo de periódico
Title: Thermal behavior of malonylglucoside isoflavones in soybean flour analyzed by RPHPLC/DAD and eletrospray ionization mass spectrometry
Author: Aguiar, Claudio L.
Haddad, Renato
Eberlin, Marcos N.
Carrao-Panizzi, Mercedes C.
Tsai, Siu M.
Park, Yong K.
Abstract: Soybean (Glycine max (Merrill) L) contains high content of aglycone isoflavones, as well as glucoside and malonylconjugates. In this work, the content of isoflavones in defatted soy flour was determined by reversed-phase high-performance liquid chromatography (RPHPLC) after alcoholic extraction in methanol/water mixture in the ratio 80:20 (v/v). It was observed that the heating treatment transformed the malonylglucosides into glucoside isoflavones. After heat treatment at 121 degrees C for 30 min, nearly all malonylisoflavones were converted into glucoside, but acetylisoflavones were not detected via RPHPLC analysis. Electrospray ionization mass spectrometry confirmed the presence of malonylisoflavones in heat-treated defatted soy flour by direct infusion analysis. (c) 2012 Elsevier Ltd. All rights reserved.
Subject: Soybean
Editor: Elsevier
Citation: Food Science and Technology. Elsevier, v.48, n.1, p.114-119, 2012
Rights: fechado
Identifier DOI: 10.1016/j.lwt.2012.02.017
Date Issue: 2012
Appears in Collections:IQ - Artigos e Outros Documentos

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