Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235895
Type: Artigo de periódico
Title: Optimization Of Amine-rich Multilayer Thin Films For The Capture And Quantification Of Prostate-specific Antigen.
Author: Polak, Roberta
Bradwell, Grinia M
Gilbert, Jonathan B
Danielsen, Scott
Beppu, Marisa M
Cohen, Robert E
Rubner, Michael F
Abstract: It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on in vitro results, these films could be potentially applied for rapid and high-throughput diagnosis of dilute biomarkers in serum or tissue. PAH presents functional amino groups that can be further reacted with desired chemistries in order to create customizable and specific surfaces for biomolecule capture. A variety of film assembly characteristics were tested (pH, molecular weight of PAH, and ionic strength) to tune the biotinylation and swelling behavior of these films to maximize detection capabilities. The resultant optimized biotinylated PAH/SPS 9.3/9.3 system was utilized in conjunction with quantum dots (Qdots) to capture and detect a dilute biomarker for prostate cancer, prostate-specific antigen (PSA). Compared to previous work, our system presents a good sensitivity for PSA detection within the clinically relevant range of 0.4-100 ng/mL.
Subject: Biomarkers, Tumor
Humans
Male
Molecular Structure
Particle Size
Photoelectron Spectroscopy
Polyamines
Polystyrenes
Prostate-specific Antigen
Prostatic Neoplasms
Quantum Dots
Sensitivity And Specificity
Citation: Langmuir : The Acs Journal Of Surfaces And Colloids. v. 31, n. 19, p. 5479-5488, 2015-May.
Rights: embargo
Identifier DOI: 10.1021/acs.langmuir.5b00443
Address: http://www.ncbi.nlm.nih.gov/pubmed/25909861
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_25909861.pdf3.06 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.