Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235243
Type: Artigo de periódico
Title: Nanofilms Of Hyaluronan/chitosan Assembled Layer-by-layer: An Antibacterial Surface For Xylella Fastidiosa.
Author: Hernández-Montelongo, Jacobo
Nascimento, Vicente F
Murillo, Duber
Taketa, Thiago B
Sahoo, Prasana
de Souza, Alessandra A
Beppu, Marisa M
Cotta, Monica A
Abstract: In this work, nanofilms of hyaluronan/chitosan (HA/CHI) assembled layer by layer were synthesized; their application as a potential antimicrobial material was demonstrated for the phytopathogen Xylella fastidiosa, a gram-negative bacterium, here used as a model. For the synthesis, the influence of pH and ionic strength of these natural polymer stem-solutions on final characteristics of the HA/CHI nanofilms was studied in detail. The antibacterial effect was evaluated using widefield fluorescence microscopy. These results were correlated with the chemical properties of the nanofilms, studied by FTIR and Raman spectroscopy, as well as with their morphology and surface properties characterized using SEM and AFM. The present findings can be extended to design and optimize HA/CHI nanofilms with enhanced antimicrobial behavior for other type of phytopathogenic gram-negative bacteria species, such as Xanthomonas citri, Xanthomas campestri and Ralstonia solanacearum.
Subject: Biopolymers
Hyaluronan/chitosan Nanofilms
Layer-by-layer
Xylella Fastidiosa
Citation: Carbohydrate Polymers. v. 136, p. 1-11, 2016-Jan.
Rights: embargo
Identifier DOI: 10.1016/j.carbpol.2015.08.076
Address: http://www.ncbi.nlm.nih.gov/pubmed/26572322
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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