Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/2292
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleAge-dependent changes in rat lacrimal gland anti-oxidant and vesicular related protein expression profilespt_BR
dc.contributor.authorBatista, Thiago Martinspt_BR
dc.contributor.authorTomiyoshi, Lilian Midoript_BR
dc.contributor.authorDias, Ana Carolinapt_BR
dc.contributor.authorRoma, Leticia Pratespt_BR
dc.contributor.authorModulo, Carolina Mariapt_BR
dc.contributor.authorMalki, Leonardo Tannuspt_BR
dc.contributor.authorMachado Filho, Elisio Buenopt_BR
dc.contributor.authorDeminice, Rafaelpt_BR
dc.contributor.authorJordao, Alceu Afonso, Jr.pt_BR
dc.contributor.authorCunha, Daniel A.pt_BR
dc.contributor.authorRocha, Eduardo Melanipt_BR
unicamp.authorBatista, Thiago Martinspt_BR
unicamp.authorRoma, Leticia Pratespt_BR
unicamp.authorCunha, Daniel A.pt_BR
unicamp.author.externalTomiyoshi, Lilian Midoript
unicamp.author.externalDias, Ana Carolinapt
unicamp.author.externalModulo, Carolina Mariapt
unicamp.author.externalMalki, Leonardo Tannuspt
unicamp.author.externalMachado Filho, Elisio Buenopt
unicamp.author.externalDeminice, Rafaelpt
unicamp.author.externalJordao, Alceu Afonso, Jr.pt
unicamp.author.externalRocha, Eduardo Melanipt
dc.subject.wosAUTOPHAGIC VACUOLESpt_BR
dc.subject.wosCALORIE RESTRICTIONpt_BR
dc.subject.wosSALIVARY-GLANDSpt_BR
dc.subject.wosOCULAR SURFACEpt_BR
dc.subject.wosSNARE COMPLEXpt_BR
dc.subject.wosACINAR-CELLSpt_BR
dc.subject.wosEXOCYTOSISpt_BR
dc.subject.wosMECHANISMSpt_BR
dc.subject.wosDISEASEpt_BR
dc.subject.wosLIVERpt_BR
dc.description.abstractPurpose: Anti-oxidation and exocytosis are important for maintaining exocrine tissue homeostasis. During aging, functional and structural alterations occur in the lacrimal gland (LG), including oxidative damage to proteins, lipids, and DNA. The aims of the present study were to determine in the aging LG: a) the effects of aging on LG structure and secretory activity and b) changes in the expression of oxidative stress markers. Methods: To address these goals, tear secretion composition and corneal impression cytology were compared between male Wistar rats of 2 (control) and 24 (aged) months. LG morphology and the expression levels of vitamin E and malonaldehyde (MDA) were evaluated to determine the anti-oxidant activity and lipid peroxidation, respectively. RT-PCR and western blot analysis were used for the analysis of Ras related in brain GTPase protein (Rab) and soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins of the secretory machinery (i.e.; Rab 3d, Rab 27, vesicle-associated membrane protein-2 (Vamp-2), and syntaxin). Results: Histological analysis of aged rats revealed a higher frequency of corneal epithelia metaplasia. In the acinar cells, organelles underwent degeneration, and lipofucsin-like material accumulated in the cytoplasm along with declines in the anti-oxidant marker vitamin E. Rab3d and Rab27b mRNA levels fell along with Rab3d protein expression, whereas syntaxin levels increased. Conclusions: These findings indicate that exocytotic and anti-oxidant mechanisms become impaired with age in the rat LG. In parallel with these structural alterations, functional declines may contribute to the pathophysiology caused by tear film modification in dry eye disease.pt
dc.relation.ispartofMolecular Visionpt_BR
dc.publisher.cityAtlantapt_BR
dc.publisher.countryUSApt_BR
dc.publisherMolecular Visionpt_BR
dc.date.issued2012pt_BR
dc.identifier.citationMolecular Vision. Molecular Vision, v.18, n.22-23, p.194-202, 2012pt_BR
dc.language.isoengpt_BR
dc.description.volume18pt_BR
dc.description.issuenumber22-23pt_BR
dc.description.firstpage194pt_BR
dc.description.lastpage202pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1090-0535pt_BR
dc.identifier.wosidWOS:000301240400001pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundacao de Apoio ao Ensino, Pesquisa e Assistencia do Hospital das Clinicas da Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo (FAEPA)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2013-09-19T18:06:36Z
dc.date.available2016-07-01T14:43:25Z-
dc.date.accessioned2013-09-19T18:06:36Z
dc.date.accessioned2016-07-01T14:43:25Z-
dc.description.provenanceMade available in DSpace on 2013-09-19T18:06:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.description.provenanceMade available in DSpace on 2016-07-01T14:43:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/2292
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/2292-
dc.contributor.departmentFisiologia e Biofísica
dc.contributor.unidadeIBpt
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