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Type: Artigo de periódico
Title: In vitro influence of the extracellular matrix in myoepithelial cells stimulated by malignant conditioned medium
Author: Martinez, Elizabeth F.
Dias Demasi, Ana Paula
Napimoga, Marcelo Henrique
Arana-Chavez, Victor Elias
Altemani, Albina
de Araujo, Ney Soares
de Araujo, Vera Cavalcanti
Abstract: In order to investigate the role of myoepithelial cell and tumor microenvironment in salivary gland neoplasma, we have performed a study towards the effect of different extracellular matrix proteins (basement membrane matrix, type I collagen and fibronectin) on morphology and differentiation of benign myoepithelial cells from pleomorphic adenoma cultured with malignant cell culture medium from squamous cell carcinoma. We have also analyzed the expression of alpha-smooth muscle actin (alpha-SMA) and FGF-2 by immunofluorescence and qPCR. Our immunofluorescence results, supported by qPCR analysis, demonstrated that alpha-SMA and FGF-2 were upregulated in the benign myoepithelial cells from pleomorphic adenoma in all studied conditions on fibronectin substratum. However, the myoepithelial cells on fibronectin substratum did not alter their morphology under malignant conditioned medium stimulation and exhibited a stellate morphology and, occasionally focal adhesions with the substratum. In summary, our data demonstrated that the extracellular matrix exerts an important role in the morphology of the benign myoepithelial cells by the presence of focal adhesions and also inducing increase FGF-2 and alpha-SMA expression by these cells, especially in the fibronectin substratum. (C) 2011 Elsevier Ltd. All rights reserved.
Subject: Myoepithelial cells
Extracellular matrix
Pleomorphic adenoma
Editor: Elsevier
Citation: Oral Oncology. Elsevier, v.48, n.2, p.102-109, 2012
Rights: fechado
Identifier DOI: 10.1016/j.oraloncology.2011.09.008
Date Issue: 2012
Appears in Collections:FCM - Artigos e Outros Documentos

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