Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Desenvolvimento e validação de método espectrofotométrico para determinação de corante à base de luteína adicionado em iogurte desnatado
Title Alternative: Development and validation of a spectrophotometric method for determination of lutein colorant added to nonfat yogurt
Author: Xavier, Ana Augusta Odorissi
Mercadante, Adriana Zerlotti
Domingos, Lígia Dozena
Viotto, Walkíria Hanada
Abstract: A simple analytical method for extraction and quantification of lutein colorant added to yogurt was developed and validated. The method allowed complete extraction of carotenoids using tetrahydrofuran in vortex, followed by centrifugation, partition to diethyl ether/petroleum ether, and drying. The carotenoids dissolved in ethanol were quantified by UV-Vis spectrophotometry. This method showed linearity in the range tested (1.41-13.42 µg g-1), limits of detection and quantification of 0.42 and 1.28 µg g-1, respectively, low relative standard deviation (3.4%) and recovery ranging from 95 to 103%. The method proved reliable for quantification of lutein added to yogurt.
Subject: UV-Vis spectroscopy
lutein colorant
Editor: Sociedade Brasileira de Química
Citation: Química Nova. Sociedade Brasileira de Química, v. 35, n. 10, p. 2057-2062, 2012.
Rights: aberto
Identifier DOI: 10.1590/S0100-40422012001000028
Date Issue: 1-Jan-2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
S0100-40422012001000028.pdf505.85 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.