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Type: Artigo de periódico
Title: Evidences Of Endocytosis Via Caveolae Following Blood-brain Barrier Breakdown By Phoneutria Nigriventer Spider Venom.
Author: Soares, Edilene Siqueira
Mendonça, Monique Culturato Padilha
Irazusta, Silvia Pierre
Coope, Andressa
Stávale, Leila Miguel
da Cruz-Höfling, Maria Alice
Abstract: Spider venoms contain neurotoxic peptides aimed at paralyzing prey or for defense against predators; that is why they represent valuable tools for studies in neuroscience field. The present study aimed at identifying the process of internalization that occurs during the increased trafficking of vesicles caused by Phoneutria nigriventer spider venom (PNV)-induced blood-brain barrier (BBB) breakdown. Herein, we found that caveolin-1α is up-regulated in the cerebellar capillaries and Purkinje neurons of PNV-administered P14 (neonate) and 8- to 10-week-old (adult) rats. The white matter and granular layers were regions where caveolin-1α showed major upregulation. The variable age played a role in this effect. Caveolin-1 is the central protein that controls caveolae formation. Caveolar-specialized cholesterol- and sphingolipid-rich membrane sub-domains are involved in endocytosis, transcytosis, mechano-sensing, synapse formation and stabilization, signal transduction, intercellular communication, apoptosis, and various signaling events, including those related to calcium handling. PNV is extremely rich in neurotoxic peptides that affect glutamate handling and interferes with ion channels physiology. We suggest that the PNV-induced BBB opening is associated with a high expression of caveolae frame-forming caveolin-1α, and therefore in the process of internalization and enhanced transcytosis. Caveolin-1α up-regulation in Purkinje neurons could be related to a way of neurons to preserve, restore, and enhance function following PNV-induced excitotoxicity. The findings disclose interesting perspectives for further molecular studies of the interaction between PNV and caveolar specialized membrane domains. It proves PNV to be excellent tool for studies of transcytosis, the most common form of BBB-enhanced permeability.
Subject: Animals
Blood-brain Barrier
Blotting, Western
Caveolin 1
Purkinje Cells
Rats, Wistar
Real-time Polymerase Chain Reaction
Spider Venoms
Lipid Rafts
Transcellular Trafficking
Citation: Toxicology Letters. v. 229, n. 3, p. 415-22, 2014-Sep.
Rights: fechado
Identifier DOI: 10.1016/j.toxlet.2014.07.018
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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