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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleQuantitative Proteomic Analysis Reveals Metabolic Alterations, Calcium Dysregulation, And Increased Expression Of Extracellular Matrix Proteins In Laminin α2 Chain-deficient Muscle.pt_BR
dc.contributor.authorde Oliveira, Bruno Menezespt_BR
dc.contributor.authorMatsumura, Cintia Ypt_BR
dc.contributor.authorFontes-Oliveira, Cibely Cpt_BR
dc.contributor.authorGawlik, Kinga Ipt_BR
dc.contributor.authorAcosta, Helenapt_BR
dc.contributor.authorWernhoff, Patrikpt_BR
dc.contributor.authorDurbeej, Madeleinept_BR
unicamp.author¶Departament of Functional and Structural Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-970, Brazil;pt_BR
unicamp.author.externalBruno Menezes de Oliveira, From the §Department of Experimental Medical Science, Unit of Muscle Biology, Lund University, BMC B12, 221 84 Lund, Sweden;pt
unicamp.author.externalCintia Y Matsumura, From the §Department of Experimental Medical Science, Unit of Muscle Biology, Lund University, BMC B12, 221 84 Lund, Swedenpt
unicamp.author.externalCibely C Fontes-Oliveira, From the §Department of Experimental Medical Science, Unit of Muscle Biology, Lund University, BMC B12, 221 84 Lund, Sweden;pt
unicamp.author.externalKinga I Gawlik, From the §Department of Experimental Medical Science, Unit of Muscle Biology, Lund University, BMC B12, 221 84 Lund, Sweden;pt
unicamp.author.externalHelena Acosta, ‖Stem Cell Center, Lund University, BMC B12, 221 84 Lund, Sweden.pt
unicamp.author.externalPatrik Wernhoff, From the §Department of Experimental Medical Science, Unit of Muscle Biology, Lund University, BMC B12, 221 84 Lund, Sweden;pt
unicamp.author.externalMadeleine Durbeej, From the §Department of Experimental Medical Science, Unit of Muscle Biology, Lund University, BMC B12, 221 84 Lund, Swedenpt
unicamp.author.externalmadeleine.durbeej-hjalt@med.lu.se.pt
dc.description.abstractCongenital muscular dystrophy with laminin α2 chain deficiency (MDC1A) is one of the most severe forms of muscular disease and is characterized by severe muscle weakness and delayed motor milestones. The genetic basis of MDC1A is well known, yet the secondary mechanisms ultimately leading to muscle degeneration and subsequent connective tissue infiltration are not fully understood. In order to obtain new insights into the molecular mechanisms underlying MDC1A, we performed a comparative proteomic analysis of affected muscles (diaphragm and gastrocnemius) from laminin α2 chain-deficient dy(3K)/dy(3K) mice, using multidimensional protein identification technology combined with tandem mass tags. Out of the approximately 700 identified proteins, 113 and 101 proteins, respectively, were differentially expressed in the diseased gastrocnemius and diaphragm muscles compared with normal muscles. A large portion of these proteins are involved in different metabolic processes, bind calcium, or are expressed in the extracellular matrix. Our findings suggest that metabolic alterations and calcium dysregulation could be novel mechanisms that underlie MDC1A and might be targets that should be explored for therapy. Also, detailed knowledge of the composition of fibrotic tissue, rich in extracellular matrix proteins, in laminin α2 chain-deficient muscle might help in the design of future anti-fibrotic treatments. All MS data have been deposited in the ProteomeXchange with identifier PXD000978 (http://proteomecentral.proteomexchange.org/dataset/PXD000978).en
dc.relation.ispartofMolecular & Cellular Proteomics : Mcppt_BR
dc.relation.ispartofabbreviationMol. Cell Proteomicspt_BR
dc.date.issued2014-Novpt_BR
dc.identifier.citationMolecular & Cellular Proteomics : Mcp. v. 13, n. 11, p. 3001-13, 2014-Nov.pt_BR
dc.language.isoengpt_BR
dc.description.volume13pt_BR
dc.description.firstpage3001-13pt_BR
dc.rightsfechadopt_BR
dc.rights.holder© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1535-9484pt_BR
dc.identifier.doi10.1074/mcp.M113.032276pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/24994560pt_BR
dc.date.available2015-11-27T13:42:52Z-
dc.date.accessioned2015-11-27T13:42:52Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:42:52Z (GMT). No. of bitstreams: 1 pmed_24994560.pdf: 1505824 bytes, checksum: 0a4379f4e755333a60bfa5a53b6f4826 (MD5) Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201530-
dc.identifier.idPubmed24994560pt_BR
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