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Type: Artigo de periódico
Title: Up-regulation Of Dnam-1 And Nkp30, Associated With Improvement Of Nk Cells Activation After Long-term Culture Of Mononuclear Cells From Patients With Ovarian Neoplasia.
Author: da Silva, Rodrigo Fernandes
Petta, Carlos Alberto
Derchain, Sophie Françoise
Alici, Evren
Guimarães, Fernando
Abstract: This study aimed at evaluating the functional activation and activating receptors expression on resting, short- and long-term NK and NK-like T cells from blood of ovarian neoplasia patients. Blood from patients with adnexal benign alterations (n = 10) and ovarian cancer (grade I-IV n = 14) were collected after signed consent. Effector cells activation was evaluated by the expression of the CD107a molecule. Short-term culture was conducted overnight with IL-2 and long-term culture for 21 days, by a method designed to expand CD56(+) lymphocytes. Short-term culture significantly increased NK cells activation compared to resting NK cells (p<0.05), however, the long-term procedure supported an even higher increase (p<0.001). Resting NK-like T cells showed poor activation, which was not altered by the culture procedures. The long-term culture effectively increased the expression of the activating receptors on NK and NK-like T cells, either by increasing the number of cells expressing a given receptor and/or by up-regulating their expression intensity. As a conclusion, the long-term culture system employed, resulted in a high number of functional NK cells. The culture system was particularly efficient on the up-regulation of NKp30 and DNAM-1 receptors on NK cells.
Subject: Cd107a
Ncrs (natural Cytotoxic Receptors)
Nk-like T Lymphocytes
Regulatory T Cells
Citation: Human Immunology. v. 75, n. 8, p. 777-84, 2014-Aug.
Rights: fechado
Identifier DOI: 10.1016/j.humimm.2014.05.010
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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