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Type: Artigo de periódico
Title: Effects Of Different Intensities Of Physical Exercise On Insulin Sensitivity And Protein Kinase B/akt Activity In Skeletal Muscle Of Obese Mice.
Author: Marinho, Rodolfo
Moura, Leandro Pereira de
Rodrigues, Bárbara de Almeida
Pauli, Luciana Santos Souza
Silva, Adelino Sanchez Ramos da
Ropelle, Eloize Cristina Chiarreotto
Souza, Claudio Teodoro de
Cintra, Dennys Esper Corrêa
Ropelle, Eduardo Rochete
Pauli, José Rodrigo
Abstract: To investigate the effects of different intensities of acute exercise on insulin sensitivity and protein kinase B/Akt activity in skeletal muscle of obese mice. Swiss mice were randomly divided into four groups, and fed either a standard diet (control group) or high fat diet (obese sedentary group and obese exercise group 1 and 2) for 12 weeks. Two different exercise protocols were used: swimming for 1 hour with or without an overload of 5% body weight. The insulin tolerance test was performed to estimate whole-body sensitivity. Western blot technique was used to determine protein levels of protein kinase B/Akt and phosphorylation by protein Kinase B/Akt in mice skeletal muscle. A single bout of exercise inhibited the high fat diet-induced insulin resistance. There was increase in phosphorylation by protein kinase B/Akt serine, improve in insulin signaling and reduce of fasting glucose in mice that swam for 1 hour without overload and mice that swan for 1 hour with overload of 5%. However, no significant differences were seen between exercised groups. Regardless of intensity, aerobic exercise was able to improve insulin sensitivity and phosphorylation by protein kinase B/Ak, and proved to be a good form of treatment and prevention of type 2 diabetes.
Subject: Animals
Blood Glucose
Blotting, Western
Diabetes Mellitus, Type 2
Diet, High-fat
Enzyme-linked Immunosorbent Assay
Insulin Resistance
Mice, Obese
Muscle, Skeletal
Physical Conditioning, Animal
Proto-oncogene Proteins C-akt
Random Allocation
Time Factors
Citation: Einstein (são Paulo, Brazil). v. 12, n. 1, p. 82-9
Rights: fechado
Appears in Collections:Unicamp - Artigos e Outros Documentos

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