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Type: Artigo de periódico
Title: Evolutionarily Conserved Morphogenetic Movements At The Vertebrate Head-trunk Interface Coordinate The Transport And Assembly Of Hypopharyngeal Structures.
Author: Lours-Calet, Corinne
Alvares, Lucia E
El-Hanfy, Amira S
Gandesha, Saniel
Walters, Esther H
Sobreira, Débora Rodrigues
Wotton, Karl R
Jorge, Erika C
Lawson, Jennifer A
Kelsey Lewis, A
Tada, Masazumi
Sharpe, Colin
Kardon, Gabrielle
Dietrich, Susanne
Abstract: The vertebrate head-trunk interface (occipital region) has been heavily remodelled during evolution, and its development is still poorly understood. In extant jawed vertebrates, this region provides muscle precursors for the throat and tongue (hypopharyngeal/hypobranchial/hypoglossal muscle precursors, HMP) that take a stereotype path rostrally along the pharynx and are thought to reach their target sites via active migration. Yet, this projection pattern emerged in jawless vertebrates before the evolution of migratory muscle precursors. This suggests that a so far elusive, more basic transport mechanism must have existed and may still be traceable today. Here we show for the first time that all occipital tissues participate in well-conserved cell movements. These cell movements are spearheaded by the occipital lateral mesoderm and ectoderm that split into two streams. The rostrally directed stream projects along the floor of the pharynx and reaches as far rostrally as the floor of the mandibular arch and outflow tract of the heart. Notably, this stream leads and engulfs the later emerging HMP, neural crest cells and hypoglossal nerve. When we (i) attempted to redirect hypobranchial/hypoglossal muscle precursors towards various attractants, (ii) placed non-migratory muscle precursors into the occipital environment or (iii) molecularly or (iv) genetically rendered muscle precursors non-migratory, they still followed the trajectory set by the occipital lateral mesoderm and ectoderm. Thus, we have discovered evolutionarily conserved morphogenetic movements, driven by the occipital lateral mesoderm and ectoderm, that ensure cell transport and organ assembly at the head-trunk interface.
Subject: Animals
Biological Evolution
Cell Movement
In Situ Hybridization
Neural Crest
Species Specificity
Circumpharyngeal Route
Evolution Of Vertebrate Developmental Mechanisms
Floor Of Pharynx
Head–trunk Interface
Hypobranchial/hypoglossal Muscle
Migratory Muscle Precursors
Morphogenetic Movements
Occipital Ectoderm
Occipital Lateral Mesoderm
Occipital Neural Crest
Occipital Somites
Pharyngeal Arches
Citation: Developmental Biology. v. 390, n. 2, p. 231-46, 2014-Jun.
Rights: fechado
Identifier DOI: 10.1016/j.ydbio.2014.03.003
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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