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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleCarotenoids Inhibit Lipid Peroxidation And Hemoglobin Oxidation, But Not The Depletion Of Glutathione Induced By Ros In Human Erythrocytes.pt_BR
dc.contributor.authorChisté, Renan Campospt_BR
dc.contributor.authorFreitas, Marisapt_BR
dc.contributor.authorMercadante, Adriana Zerlottipt_BR
dc.contributor.authorFernandes, Eduardapt_BR
unicamp.authorRenan Campos Chisté, Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, SP, Brazilpt_BR
unicamp.authorAdriana Zerlotti Mercadante, Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, SP, Brazil.pt_BR
unicamp.author.externalREQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), 4050-313 Porto, Portugal. Electronic address: rcchiste@ff.up.pt.pt
unicamp.author.externalMarisa Freitas, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), 4050-313 Porto, Portugal.pt
unicamp.author.externalEduarda Fernandes, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), 4050-313 Porto, Portugal. Electronic address: egracas@ff.up.pt.pt
dc.subjectAntioxidantspt_BR
dc.subjectBiological Markerspt_BR
dc.subjectCarotenoidspt_BR
dc.subjectErythrocytespt_BR
dc.subjectGlutathionept_BR
dc.subjectHemoglobinspt_BR
dc.subjectHumanspt_BR
dc.subjectInhibitory Concentration 50pt_BR
dc.subjectLipid Peroxidationpt_BR
dc.subjectMolecular Structurept_BR
dc.subjectOxidation-reductionpt_BR
dc.subjectReactive Oxygen Speciespt_BR
dc.subjectAntioxidant Capacitypt_BR
dc.subjectBioactive Compoundspt_BR
dc.subjectOxidative Stresspt_BR
dc.subjectPeroxyl Radicalspt_BR
dc.subjectReactive Oxygen Speciespt_BR
dc.description.abstractDespite the presence of endogenous antioxidants in erythrocytes, these cells are highly susceptible to oxidative damage and some exogenous antioxidants, such as carotenoids, are able to inhibit the pro-oxidant effect provided by reactive oxygen species. In this study, we evaluated the potential of carotenoids usually detected in human blood plasma (β-carotene, zeaxanthin, lutein, β-cryptoxanthin and lycopene) to prevent the oxidative damage in erythrocytes. Human erythrocytes were subjected to induced oxidative damage and the following biomarkers of oxidative stress were monitored: lipid peroxidation [induced by tert-butyl hydroperoxide (tBHP) or by 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH)] and AAPH-induced oxidation of hemoglobin and depletion of glutathione. When tBHP was used to induce lipid peroxidation, lycopene was the most efficient carotenoid (IC50=2.2 ± 0.4 μM), while lutein was the most efficient (IC50=2.5 ± 0.7 μM) when peroxyl radicals (ROO) were generated by AAPH. In relation to the hemoglobin oxidation induced by AAPH, β-carotene and zeaxanthin were the most efficient antioxidants (IC50=2.9 ± 0.3 μM and 2.9 ± 0.1 μM, respectively). Surprisingly β-cryptoxanthin and lycopene did not inhibit hemoglobin oxidation or lipid peroxidation when induced by AAPH, even at the highest tested concentration (3 μM). Additionally, the tested carotenoids did not prevent ROO-mediated GSH depletion and GSSG formation probably due to the lack of interaction between carotenoids (apolar) and glutathione (polar). Our study contributes with important insights that carotenoids may exert therapeutical potential to act as a natural antioxidant to prevent ROO-induced toxicity in human erythrocytes.en
dc.relation.ispartofLife Sciencespt_BR
dc.relation.ispartofabbreviationLife Sci.pt_BR
dc.date.issued2014-Marpt_BR
dc.identifier.citationLife Sciences. v. 99, n. 1-2, p. 52-60, 2014-Mar.pt_BR
dc.language.isoengpt_BR
dc.description.volume99pt_BR
dc.description.firstpage52-60pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2014 Elsevier Inc. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1879-0631pt_BR
dc.identifier.doi10.1016/j.lfs.2014.01.059pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/24486304pt_BR
dc.date.available2015-11-27T13:41:52Z-
dc.date.accessioned2015-11-27T13:41:52Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:41:52Z (GMT). No. of bitstreams: 1 pmed_24486304.pdf: 694143 bytes, checksum: b95604c0eac9670a628cff1bd80c051b (MD5) Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201117-
dc.identifier.idPubmed24486304pt_BR
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