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Type: Artigo de periódico
Title: Arhgap21 Protein, A New Partner Of α-tubulin Involved In Cell-cell Adhesion Formation And Essential For Epithelial-mesenchymal Transition.
Author: Barcellos, Karin S A
Bigarella, Carolina L
Wagner, Mark V
Vieira, Karla P
Lazarini, Mariana
Langford, Peter R
Machado-Neto, João A
Call, Steven G
Staley, Davis M
Chung, Jarom Y
Hansen, Marc D
Saad, Sara T O
Abstract: Cell-cell adhesions and the cytoskeletons play important and coordinated roles in cell biology, including cell differentiation, development, and migration. Adhesion and cytoskeletal dynamics are regulated by Rho-GTPases. ARHGAP21 is a negative regulator of Rho-GTPases, particularly Cdc42. Here we assess the function of ARHGAP21 in cell-cell adhesion, cell migration, and scattering. We find that ARHGAP21 is localized in the nucleus, cytoplasm, or perinuclear region but is transiently redistributed to cell-cell junctions 4 h after initiation of cell-cell adhesion. ARHGAP21 interacts with Cdc42, and decreased Cdc42 activity coincides with the appearance of ARHGAP21 at the cell-cell junctions. Cells lacking ARHGAP21 expression show weaker cell-cell adhesions, increased cell migration, and a diminished ability to undergo hepatocyte growth factor-induced epithelial-mesenchymal transition (EMT). In addition, ARHGAP21 interacts with α-tubulin, and it is essential for α-tubulin acetylation in EMT. Our findings indicate that ARHGAP21 is a Rho-GAP involved in cell-cell junction remodeling and that ARHGAP21 affects migration and EMT through α-tubulin interaction and acetylation.
Subject: Acetylation
Cell Adhesion
Cell Communication
Cell Line, Tumor
Cell Movement
Epithelial-mesenchymal Transition
Gtpase-activating Proteins
Madin Darby Canine Kidney Cells
Neoplasm Metastasis
Rna Interference
Time Factors
Cdc42 Gtp-binding Protein
Citation: The Journal Of Biological Chemistry. v. 288, n. 4, p. 2179-89, 2013-Jan.
Rights: fechado
Identifier DOI: 10.1074/jbc.M112.432716
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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