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Type: Artigo de periódico
Title: Effects Of Zoledronic Acid On Odontoblast-like Cells.
Author: Basso, F G
Turrioni, A P S
Hebling, J
de Souza Costa, C A
Abstract: The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5×10(4)cells/cm(2)) were seeded for 48h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24h, ZOL (1 or 5μM) was added to the medium and maintained in contact with the cells for 24h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability - MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1μM ZOL caused a significant increase in Col-I expression. Although 5μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p<0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex.
Subject: Alkaline Phosphatase
Bone Density Conservation Agents
Cell Survival
Cells, Cultured
Collagen Type I
Dose-response Relationship, Drug
Extracellular Matrix Proteins
Gene Expression Regulation, Enzymologic
Protein Synthesis Inhibitors
Succinate Dehydrogenase
Citation: Archives Of Oral Biology. v. 58, n. 5, p. 467-73, 2013-May.
Rights: fechado
Identifier DOI: 10.1016/j.archoralbio.2012.09.016
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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