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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleInvolvement Of Nitrite In The Nitrate-mediated Modulation Of Fermentative Metabolism And Nitric Oxide Production Of Soybean Roots During Hypoxia.pt_BR
dc.contributor.authorOliveira, Halley Cpt_BR
dc.contributor.authorSalgado, Ionept_BR
dc.contributor.authorSodek, Ladaslavpt_BR
unicamp.authorHalley C Oliveira, Department of Plant Biology, Institute of Biology, University of Campinas-UNICAMP, CP 6109 Campinas, SP 13083-970, Brazil. halleyco@unicamp.brpt_BR
unicamp.author.externalIone Salgado,pt
unicamp.author.externalLadaslav Sodek,pt
dc.subjectBenzoatespt_BR
dc.subjectCarboxylic Acidspt_BR
dc.subjectFermentationpt_BR
dc.subjectGas Chromatography-mass Spectrometrypt_BR
dc.subjectImidazolespt_BR
dc.subjectLactatespt_BR
dc.subjectNitratespt_BR
dc.subjectNitric Oxidept_BR
dc.subjectNitric Oxide Donorspt_BR
dc.subjectNitritespt_BR
dc.subjectOxygenpt_BR
dc.subjectPlant Rootspt_BR
dc.subjectQuaternary Ammonium Compoundspt_BR
dc.subjectSoybeanspt_BR
dc.subjectSuccinatespt_BR
dc.subjectTime Factorspt_BR
dc.subjectTriazenespt_BR
dc.description.abstractIt is widely accepted that nitrate but not ammonium improves tolerance of plants to hypoxic stress, although the mechanisms related to this beneficial effect are not well understood. Recently, nitrite derived from nitrate reduction has emerged as the major substrate for the synthesis of nitric oxide (NO), an important signaling molecule in plants. Here, we analyzed the effect of different nitrogen sources (nitrate, nitrite and ammonium) on the metabolic response and NO production of soybean roots under hypoxia. Organic acid analysis showed that root segments isolated from nitrate-cultivated plants presented a lower accumulation of lactate and succinate in response to oxygen deficiency in relation to those from ammonium-cultivated plants. The more pronounced lactate accumulation by root segments of ammonium-grown plants was followed by a higher ethanol release in the medium, evidencing a more intense fermentation under oxygen deficiency than those from nitrate-grown plants. As expected, root segments from nitrate-cultivated plants produced higher amounts of nitrite and NO during hypoxia compared to ammonium cultivation. Exogenous nitrite supplied during hypoxia reduced both ethanol and lactate production and stimulated cyanide-sensitive NO emission by root segments from ammonium-cultivated plants, independent of nitrate. On the other hand, treatments with a NO donor or a NO scavenger did not affect the intensity of fermentation of soybean roots. Overall, these results indicate that nitrite participates in the nitrate-mediated modulation of the fermentative metabolism of soybean roots during oxygen deficiency. The involvement of mitochondrial reduction of nitrite to NO in this mechanism is discussed.en
dc.relation.ispartofPlantapt_BR
dc.relation.ispartofabbreviationPlantapt_BR
dc.date.issued2013-Janpt_BR
dc.identifier.citationPlanta. v. 237, n. 1, p. 255-64, 2013-Jan.pt_BR
dc.language.isoengpt_BR
dc.description.volume237pt_BR
dc.description.firstpage255-64pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1432-2048pt_BR
dc.identifier.doi10.1007/s00425-012-1773-0pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/23011570pt_BR
dc.date.available2015-11-27T13:32:02Z-
dc.date.accessioned2015-11-27T13:32:02Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:32:02Z (GMT). No. of bitstreams: 1 pmed_23011570.pdf: 564521 bytes, checksum: 2ddd4893f39219dff9855a6c12ee100b (MD5) Previous issue date: 2013en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200764-
dc.identifier.idPubmed23011570pt_BR
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