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Type: Artigo de periódico
Title: Genetic Polymorphisms And Surface Expression Of Ctla-4 And Pd-1 On T Cells Of Silica-exposed Workers.
Author: Rocha, Michelle C
Santos, Leonilda M B
Bagatin, Ericson
Cohen Tervaert, Jan W
Damoiseaux, Jan G M C
Lido, Alessandro V
Longhini, Ana L
Torello, Cristiane O
Queiroz, Mary L S
Abstract: Exposure to silica dust has been examined as a possible risk factor for autoimmune diseases, including scleroderma, rheumatoid arthritis and systemic lupus erythematosus. Since CTLA-4 [CD152] and PD-1 [CD279] are important for the maintenance of peripheral tolerance by regulating T cell responsiveness, we evaluated the expression of these molecules on the surface of CD4 and CD8 T cells, as well as single nucleotide polymorphisms (SNP) in CTLA-4 and PDCD1 genes, of 70 silica-exposed workers and 30 non-exposed, age-, ethnically- and sex-matched controls. Expression of CTLA-4 was significantly (P<0.05) reduced in CD4 T cells of exposed individuals [median=0.1% and interquartile range, IQR 0.0-0.1% (exposed), median=0.20%, IQR 0.0-0.4% (control)]. Also the expression of PD-1 was significantly (P<0.0001) reduced in both CD4 [median=0.9%, IQR 0.4-2.3% (exposed), median=5.7%, IQR 1.4-13.3% (control)] and CD8 T cells [median=0.9%, IQR 0.3-1.9% (exposed), median=5.0%, IQR 3.4-8.9% (control)]. The study of polymorphisms demonstrated a lower frequency of the A allele in the analysis of the PD1.3 SNP in the exposed group, which might be associated with the lower expression of PD-1 on the surface of CD4 T cells. Our findings provide evidence for the association of silica exposure and the maintenance of self-tolerance, i.e., the susceptibility to autoimmune disorders.
Subject: Aged
Cd4 Lymphocyte Count
Ctla-4 Antigen
Case-control Studies
Flow Cytometry
Middle Aged
Occupational Exposure
Polymorphism, Single Nucleotide
Programmed Cell Death 1 Receptor
Silicon Dioxide
Citation: International Journal Of Hygiene And Environmental Health. v. 215, n. 6, p. 562-9, 2012-Nov.
Rights: fechado
Identifier DOI: 10.1016/j.ijheh.2011.10.010
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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