Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200182
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleKnocking Down Low Molecular Weight Protein Tyrosine Phosphatase (lmw-ptp) Reverts Chemoresistance Through Inactivation Of Src And Bcr-abl Proteins.pt_BR
dc.contributor.authorFerreira, Paula Apt_BR
dc.contributor.authorRuela-de-Sousa, Roberta Rpt_BR
dc.contributor.authorQueiroz, Karla C Spt_BR
dc.contributor.authorSouza, Ana Carolina Spt_BR
dc.contributor.authorMilani, Renatopt_BR
dc.contributor.authorPilli, Ronaldo Aloisept_BR
dc.contributor.authorPeppelenbosch, Maikel Ppt_BR
dc.contributor.authorden Hertog, Jeroenpt_BR
dc.contributor.authorFerreira, Carmen Vpt_BR
unicamp.authorPaula A Ferreira, Laboratory of Bioassays and Signal Transduction, Biochemistry Department, Biology Institute, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.pt_BR
unicamp.author.externalRoberta R Ruela-de-Sousa,pt
unicamp.author.externalKarla C S Queiroz,pt
unicamp.author.externalAna Carolina S Souza,pt
unicamp.author.externalRenato Milani,pt
unicamp.author.externalRonaldo Aloise Pilli,pt
unicamp.author.externalMaikel P Peppelenbosch,pt
unicamp.author.externalJeroen den Hertog,pt
unicamp.author.externalCarmen V Ferreira,pt
dc.subjectAntineoplastic Agentspt_BR
dc.subjectCaspase 3pt_BR
dc.subjectCell Line, Tumorpt_BR
dc.subjectCell Survivalpt_BR
dc.subjectDrug Resistance, Neoplasmpt_BR
dc.subjectFusion Proteins, Bcr-ablpt_BR
dc.subjectGene Expression Regulation, Leukemicpt_BR
dc.subjectHumanspt_BR
dc.subjectK562 Cellspt_BR
dc.subjectMolecular Weightpt_BR
dc.subjectP-glycoproteinpt_BR
dc.subjectPhosphorylationpt_BR
dc.subjectProtein Tyrosine Phosphatasespt_BR
dc.subjectSrc-family Kinasespt_BR
dc.description.abstractThe development of multidrug resistance (MDR) limits the efficacy of continuous chemotherapeutic treatment in chronic myelogenous leukemia (CML). Low molecular weight protein tyrosine phosphatase (LMW-PTP) is up-regulated in several cancers and has been associated to poor prognosis. This prompted us to investigate the involvement of LMW-PTP in MDR. In this study, we investigated the role of LMW-PTP in a chemoresistant CML cell line, Lucena-1. Our results showed that LMW-PTP is highly expressed and 7-fold more active in Lucena-1 cells compared to K562 cells, the non-resistant cell line. Knocking down LMW-PTP in Lucena-1 cells reverted chemoresistance to vincristine and imatinib mesylate, followed by a decrease of Src and Bcr-Abl phosphorylation at the activating sites, inactivating both kinases. On the other hand, overexpression of LMW-PTP in K562 cells led to chemoresistance to vincristine. Our findings describe, for the first time, that LMW-PTP cooperates with MDR phenotype, at least in part, through maintaining Src and Bcr-Abl kinases in more active statuses. These findings suggest that inhibition of LMW-PTP may be a useful strategy for the development of therapies for multidrug resistant CML.en
dc.relation.ispartofPlos Onept_BR
dc.relation.ispartofabbreviationPLoS ONEpt_BR
dc.date.issued2012pt_BR
dc.identifier.citationPlos One. v. 7, n. 9, p. e44312, 2012.pt_BR
dc.language.isoengpt_BR
dc.description.volume7pt_BR
dc.description.firstpagee44312pt_BR
dc.rightsabertopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.doi10.1371/journal.pone.0044312pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/22957062pt_BR
dc.date.available2015-11-27T13:28:52Z-
dc.date.accessioned2015-11-27T13:28:52Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:28:52Z (GMT). No. of bitstreams: 1 pmed_22957062.pdf: 362399 bytes, checksum: 51b08d43066b49b3a4fe3a0acf146e87 (MD5) Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200182-
dc.identifier.idPubmed22957062pt_BR
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