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Type: Artigo de periódico
Title: Chitosan-solid Lipid Nanoparticles As Carriers For Topical Delivery Of Tretinoin.
Author: Ridolfi, Daniela M
Marcato, Priscyla D
Justo, Giselle Z
Cordi, Lívia
Machado, Daisy
Durán, Nelson
Abstract: Tretinoin (TRE) or all-trans retinoic acid is employed in the topical treatment of various skin diseases including acne and psoriasis. However, its use is strongly limited by side effects and high chemical instability. TRE encapsulation in nanostructured systems reduces these problems. Chitosan is a biopolymer that exhibits a number of interesting properties such as bioadhesion and antibacterial activity. The aim of this work was to prepare and characterize solid lipid nanoparticles (SLN) containing TRE, with and without addition of chitosan, to assess their in vitro cytotoxicity in keratinocytes and to evaluate their antibacterial activity against bacteria related to acne. SLN without (SLN-TRE) and with (SLN-chitosan-TRE) chitosan were prepared by hot high pressure homogenization. The hydrodynamic mean diameter and zeta potential were 162.7±1.4 nm and -31.9±2.0 mV for SLN-TRE, and 284.8±15.0 nm and 55.9±3.1 mV for SLN-chitosan-TRE. The SLN-chitosan-TRE exhibited high encapsulation efficiency, high physical stability in the tested period (one year), were not cytotoxic to keratinocytes and showed high antibacterial activity against P. acnes and S. aureus. Therefore chitosan-SLN can be good candidates to encapsulate TRE and to increase its therapeutic efficacy in the topical treatment of acne.
Subject: Acne Vulgaris
Administration, Topical
Cell Survival
Cells, Cultured
Drug Carriers
Drug Compounding
Drug Stability
Microbial Sensitivity Tests
Microscopy, Electron, Transmission
Particle Size
Propionibacterium Acnes
Staphylococcus Aureus
Citation: Colloids And Surfaces. B, Biointerfaces. v. 93, p. 36-40, 2012-May.
Rights: fechado
Identifier DOI: 10.1016/j.colsurfb.2011.11.051
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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