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Type: Artigo de periódico
Title: Recognition Of Serous Ovarian Tumors In Human Samples By Multimodal Nonlinear Optical Microscopy.
Author: Adur, Javier
Pelegati, Vitor B
Costa, Leverson F L
Pietro, Luciana
de Thomaz, Andre A
Almeida, Diogo B
Bottcher-Luiz, Fatima
Andrade, Liliana A L A
Cesar, Carlos L
Abstract: We used a multimodal nonlinear optics microscopy, specifically two-photon excited fluorescence (TPEF), second and third harmonic generation (SHG∕THG) microscopies, to observe pathological conditions of ovarian tissues obtained from human samples. We show that strong TPEF + SHG + THG signals can be obtained in fixed samples stained with hematoxylin and eosin (H&E) stored for a very long time, and that H&E staining enhanced the THG signal. We then used the multimodal TPEF-SHG-THG microscopies in a stored file of H&E stained samples of human ovarian cancer to obtain complementary information about the epithelium∕stromal interface, such as the transformation of epithelium surface (THG) and the overall fibrillary tissue architecture (SHG). This multicontrast nonlinear optics microscopy is able to not only differentiate between cancerous and healthy tissue, but can also distinguish between normal, benign, borderline, and malignant specimens according to their collagen disposition and compression levels within the extracellular matrix. The dimensions of the layers of epithelia can also be measured precisely and automatically. Our data demonstrate that optical techniques can detect pathological changes associated with ovarian cancer.
Subject: Adult
Analysis Of Variance
Cystadenocarcinoma, Serous
Epithelial Cells
Equipment Design
Image Processing, Computer-assisted
Microscopy, Fluorescence, Multiphoton
Middle Aged
Ovarian Neoplasms
Citation: Journal Of Biomedical Optics. v. 16, n. 9, p. 096017, 2011-Sep.
Rights: aberto
Identifier DOI: 10.1117/1.3626575
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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