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Type: Artigo de periódico
Title: Sildenafil Preserves Diastolic Relaxation After Reduction By L-name And Increases Phosphodiesterase-5 In The Intercalated Discs Of Cardiac Myocytes And Arterioles.
Author: Ferreira-Melo, Silvia Elaine
Demacq, Caroline
Lacchini, Silvia
Krieger, José Eduardo
Irigoyen, Maria Cláudia
Moreno, Heitor
Abstract: We investigated the influence of sildenafil on cardiac contractility and diastolic relaxation and examined the distribution of phosphodiesterase-5 in the hearts of hypertensive rats that were treated with by NG-nitro-L-arginine methyl ester (L-NAME). Male Wistar rats were treated with L-NAME and/or sildenafil for eight weeks. The Langendorff method was used to examine the effects of sildenafil on cardiac contractility and diastolic relaxation. The presence and location of phosphodiesterase-5 and phosphodiesterase-3 were assessed by immunohistochemistry, and cGMP plasma levels were measured by ELISA. In isolated hearts, sildenafil prevented the reduction of diastolic relaxation (dP/dt) that was induced by L-NAME. In addition, phosphodiesterase-5 immunoreactivity was localized in the intercalated discs between the myocardial cells. The staining intensity was reduced by L-NAME, and sildenafil treatment abolished this reduction. Consistent with these results, the plasma levels of cGMP were decreased in the L-NAME-treated rats but not in rats that were treated with L-NAME + sildenafil. The sildenafil-induced attenuation of the deleterious hemodynamic and cardiac morphological effects of L-NAME in cardiac myocytes is mediated (at least in part) by the inhibition of phosphodiesterase-5.
Subject: Animals
Blood Pressure
Cyclic Nucleotide Phosphodiesterases, Type 5
Enzyme Inhibitors
Enzyme-linked Immunosorbent Assay
Myocytes, Cardiac
Ng-nitroarginine Methyl Ester
Phosphodiesterase 5 Inhibitors
Rats, Wistar
Time Factors
Citation: Clinics (são Paulo, Brazil). v. 66, n. 7, p. 1253-8, 2011.
Rights: aberto
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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