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Type: Artigo de periódico
Title: Characterization Of The Urinary Bladder Dysfunction In Renovascular Hypertensive Rats.
Author: Ramos-Filho, Antonio C S
Mónica, Fabíola Z T
Franco-Penteado, Carla F
Rojas-Moscoso, Julio A
Báu, Fernando R
Schenka, André A
De Nucci, Gilberto
Antunes, Edson
Abstract: Association between arterial hypertension and urinary bladder dysfunction has been reported in humans and spontaneously hypertensive rats. However, no study exists evaluating the bladder dysfunction in conditions of renovascular hypertension. The purpose of this study was to characterize the bladder dysfunction in two kidney-one clip (2K-1C) hypertensive rats. A silver clip was placed around the renal artery of male Wistar rats. After 8 weeks, cystometric study, concentration-response curves to contractile and relaxant agents, frequency-dependent contractions, histomorphometry, muscarinic M(2) /M(3) mRNA expression and cyclic AMP measurements were performed. 2K-1C rats showed enhanced bladder volume, wall thickness and smooth muscle density. 2K-1C rats also exhibited increases in bladder capacity and non-void contractions, and decreases in the inter-contraction intervals. In isolated detrusor smooth muscle (DSM), contractions to carbachol and electrical-field stimulation (EFS) were significantly greater in 2K-1C rats. The Rho-kinase inhibitor Y27632 (10 µM) significantly reduced the carbachol-induced contractions in SHAM and 2K-1C rats, but DSM remained overactive in 2K-1C rats in presence of Y27632. Concentration-dependent contractions to the P2X receptor agonist α,β-methylene ATP, KCl and extracellular Ca(2+) did not change between SHAM and 2K-1C groups. In 2K-1C rats, isoproterenol, metaproterenol and BRL 37-344 (non-selective, β(2) - and β(3) -selective adrenoceptor agonists, respectively) produced significantly lower relaxations and decreased cAMP levels, whereas relaxant responses to sodium nitroprusside and BAY 41-2272 remained unchanged. Muscarinic M(3) mRNA expression receptors were higher in 2K-1C group. Renovascular hypertensive rats exhibit bladder dysfunction that involves tissue remodeling and enhanced muscarinic M(3) -mediated contractions associated with reduced β-adrenoceptor-mediated signal transduction.
Subject: Adrenergic Beta-agonists
Cholinergic Agonists
Cyclic Amp
Disease Models, Animal
Dose-response Relationship, Drug
Electric Stimulation
Hypertension, Renovascular
Muscle Contraction
Muscle Relaxation
Muscle, Smooth
Neuromuscular Agents
Protein Kinase Inhibitors
Purinergic P2x Receptor Agonists
Rna, Messenger
Rats, Wistar
Receptor, Muscarinic M3
Signal Transduction
Time Factors
Urinary Bladder
Urinary Bladder Diseases
Rho-associated Kinases
Citation: Neurourology And Urodynamics. v. 30, n. 7, p. 1392-402, 2011-Sep.
Rights: fechado
Identifier DOI: 10.1002/nau.21074
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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