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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePresynaptic Action Of Bothriopsis Bilineata Smargadina (forest Viper) Venom In vitro.pt_BR
dc.contributor.authorRodrigues-Simioni, Léapt_BR
dc.contributor.authorFloriano, Rafael Stuanipt_BR
dc.contributor.authorRostelato-Ferreira, Sandropt_BR
dc.contributor.authorSousa, Norma Cristinapt_BR
dc.contributor.authorMarangoni, Sergiopt_BR
dc.contributor.authorPonce-Soto, Luis Albertopt_BR
dc.contributor.authorCarregari, Victor Corasollapt_BR
dc.contributor.authorHyslop, Stephenpt_BR
unicamp.authorLéa Rodrigues-Simioni, Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), PO Box 6111, 13083-970 Campinas, SP, Brazil. simioni@unicamp.brpt_BR
unicamp.author.externalRafael Stuani Floriano,pt
unicamp.author.externalSandro Rostelato-Ferreira,pt
unicamp.author.externalNorma Cristina Sousa,pt
unicamp.author.externalSergio Marangoni,pt
unicamp.author.externalLuis Alberto Ponce-Soto,pt
unicamp.author.externalVictor Corasolla Carregari,pt
unicamp.author.externalStephen Hyslop,pt
dc.subjectAcetylcholinept_BR
dc.subjectAnimalspt_BR
dc.subjectCarbacholpt_BR
dc.subjectChickenspt_BR
dc.subjectCreatine Kinasept_BR
dc.subjectDiaphragmpt_BR
dc.subjectIn Vitro Techniquespt_BR
dc.subjectMembrane Potentialspt_BR
dc.subjectMicept_BR
dc.subjectMuscle Contractionpt_BR
dc.subjectNeuromuscular Blocking Agentspt_BR
dc.subjectNeuromuscular Junctionpt_BR
dc.subjectPhrenic Nervept_BR
dc.subjectSynaptic Transmissionpt_BR
dc.subjectViper Venomspt_BR
dc.subjectViperidaept_BR
dc.description.abstractIn this work, we examined the neuromuscular activity of Bothriopsis bilineata smargadina (forest viper) venom in vertebrate isolated nerve-muscle preparations. In chick biventer cervicis preparations the venom caused concentration-dependent (0.1-30 μg/ml) neuromuscular blockade that was not reversed by washing, with 50% blockade occurring in 15-90 min. Muscle contractures to exogenous acetylcholine and KCl were unaffected by venom, but there was a slight increase in creatine kinase release after 120 min (from 80 ± 15 to 206 ± 25U/ml, n=6, p<0.05). In mouse phrenic nerve-diaphragm preparations, the venom (1, 10 and 30 μg/ml) produced marked facilitation (∼120% increase above basal) at the highest concentration followed by neuromuscular blockade; the effects at lower concentrations were considerably less marked. Venom increased the quantal content values after 15 and 30 min followed by significant inhibition at ≥ 90 min. However, venom did not alter the muscle membrane resting potential or the response to exogenous carbachol. In both preparations, incubation at 22 °C instead of 37 °C delayed the onset of blockade, as did inhibition of venom PLA(2) activity. In curarized mouse preparations, the venom produced only muscle facilitation. These results indicate that B. b. smargadina venom causes neuromuscular blockade in vitro by a presynaptic mechanism involving PLA(2).en
dc.relation.ispartofToxicon : Official Journal Of The International Society On Toxinologypt_BR
dc.relation.ispartofabbreviationToxiconpt_BR
dc.date.issued2011-Julpt_BR
dc.identifier.citationToxicon : Official Journal Of The International Society On Toxinology. v. 58, n. 1, p. 140-5, 2011-Jul.pt_BR
dc.language.isoengpt_BR
dc.description.volume58pt_BR
dc.description.firstpage140-5pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2011 Elsevier Ltd. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1879-3150pt_BR
dc.identifier.doi10.1016/j.toxicon.2011.05.002pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21586301pt_BR
dc.date.available2015-11-27T13:21:26Z-
dc.date.accessioned2015-11-27T13:21:26Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:21:26Z (GMT). No. of bitstreams: 1 pmed_21586301.pdf: 483409 bytes, checksum: 9034ca111a8d3fd9e2ca1ea8dc6eddc4 (MD5) Previous issue date: 2011en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199434-
dc.identifier.idPubmed21586301pt_BR
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